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验证数据展示

  • IF Staining of SH-SY5Y using CL488-15661

    IF Staining of SH-SY5Y using CL488-15661

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using CoraLite® Plus 488 Islet 1 antibody (CL488-15661) at dilution of 1:200, CL594-Phalloidin (red).

  • FC experiment of SH-SY5Y using CL488-15661

    FC experiment of SH-SY5Y using CL488-15661

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human Islet 1 (CL488-15661) (red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000, Clone: ) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

CoraLite® Plus 488-conjugated Islet 1 Polyclonal antibody

Islet 1 Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-15661

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra)

Synonyms

ISL1, Islet-1, ISLET1, ISL LIM homeobox 1, Isl 1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inSH-SY5Y cells
Positive FC (Intra) detected inSH-SY5Y cells
For other applications, we recommend the unconjugated version of this antibody, 15661-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-15661 targets Islet 1 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human
Immunogen Islet 1 fusion protein Ag8088 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ISL LIM homeobox 1
Synonyms ISL1, Islet-1, ISLET1, ISL LIM homeobox 1, Isl 1
Calculated Molecular Weight 39 kDa
Observed Molecular Weight 39-45 kDa
GenBank Accession NumberBC031213
Gene Symbol Islet 1
Gene ID (NCBI) 3670
RRIDAB_3672636
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP61371
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ISL1 belongs to the LIM homeodomain transcription factor family that binds to the enhancer region of the INS gene. It plays a pivotal role in cardiogenesis, and lacking ISL1 expression results in growth arrest or displays profound defects in heart development, including atria, ventricle, and the inflow and outflow tracts.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Islet 1 antibody CL488-15661Download protocol
FC protocol for CL Plus 488 Islet 1 antibody CL488-15661Download protocol
Standard Protocols
Click here to view our Standard Protocols