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验证数据展示

  • IF Staining of HepG2 using CL647-14600

    IF Staining of HepG2 using CL647-14600

    Immunofluorescent analysis of (-20°C Ethanol) fixed Chloroquine treated HepG2 cells using CoraLite® Plus 647 LC3 antibody (CL647-14600) at dilution of 1:100.

  • FC experiment of HeLa using CL647-14600

    FC experiment of HeLa using CL647-14600

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 647 Anti-Human LC3 (CL647-14600) (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 647-conjugated LC3 Polyclonal antibody

LC3 Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL647-14600

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC, FC (Intra)

别名

MAP1LC3B, MAP1A/MAP1B LC3 B, MAP1A/1BLC3, MAP1 light chain 3-like protein 2, LC3B

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 647
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inChloroquine treated HepG2 cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy LC3 Ready-To-Use IHC Kit. LC3 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 14600-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-14600 targets LC3 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 LC3 fusion protein Ag6144 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 microtubule-associated protein 1 light chain 3 beta
别名 MAP1LC3B, MAP1A/MAP1B LC3 B, MAP1A/1BLC3, MAP1 light chain 3-like protein 2, LC3B
计算分子量 15 kDa
GenBank蛋白编号BC067797
基因名称 LC3B
Gene ID (NCBI) 81631
ENSEMBL Gene IDENSG00000140941
RRIDAB_2920226
偶联类型 CoraLite® Plus 647 Fluorescent Dye
最大激发/发射波长654 nm / 674 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9GZQ8
储存缓冲液 PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Map1LC3, also known as LC3, is the human homolog of yeast Atg8 and is involved in the formation of autophagosomal vacuoles, called autophagosomes. Three human Map1LC3 isoforms, MAP1LC3A, MAP1LC3B, and MAP1LC3C, undergo post-translational modifications during autophagy. And they differ in their post-translation modifications during autophagy. Map1LC3 also exists in two modified forms, an 18 kDa cytoplasmic form that was originally identified as a subunit of the microtubule-associated protein 1, and a 14-16 kDa form that is associated with the autophagosome membrane. This antibody can cross react with MAP1LC3A, MAP1LC3B, and MAP1LC3C.

实验方案

Product Specific Protocols
IF protocol for CL Plus 647 LC3 antibody CL647-14600Download protocol
FC protocol for CL Plus 647 LC3 antibody CL647-14600Download protocol
Standard Protocols
Click here to view our Standard Protocols