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验证数据展示

  • IF Staining of HeLa using 14600-1-AP

    IF Staining of HeLa using 14600-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed Chloroquine treated HeLa cells using LC3 antibody (14600-1-AP) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • WB analysis of HeLa using 14600-1-AP

    WB analysis of HeLa using 14600-1-AP

    WB result of LC3 antibody (14600-1-AP; 1:6000; room temperature for 1.5 hours) with negative Control and LC3 knockout HeLa cells.

  • IP experiment of HeLa using 14600-1-AP

    IP experiment of HeLa using 14600-1-AP

    IP result of anti-LC3 (IP:14600-1-AP, 4ug; Detection:14600-1-AP 1:3000) with HeLa cells lysate 1600 ug.

  • IHC staining of human liver using 14600-1-AP

    IHC staining of human liver using 14600-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14600-1-AP (LC3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with LC3 antibody included
  • IHC staining of human liver using 14600-1-AP

    IHC staining of human liver using 14600-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14600-1-AP (LC3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with LC3 antibody included
  • FC experiment of HeLa using 14600-1-AP

    FC experiment of HeLa using 14600-1-AP

    1X10^6 HeLa cells were intracellularly stained with 0.5 ug Anti-Human LC3 (14600-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.5 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • WB analysis using 14600-1-AP

    WB analysis using 14600-1-AP

    Non-treated and Chloroquine treated HeLa cells were subjected to SDS PAGE followed by western blot with 14600-1-AP (LC3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • WB analysis using 14600-1-AP

    WB analysis using 14600-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14600-1-AP (LC3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 14600-1-AP

    IHC staining of mouse brain using 14600-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14600-1-AP (LC3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with LC3 antibody included
  • Featured Product
  • KD/KO Validated

LC3 Polyclonal antibody

LC3 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Cat No. 14600-1-AP

产品说明书

引用文献(2431)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (6)

应用

WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (2)

别名

MAP1LC3B, ATG8F, Autophagy-related ubiquitin-like modifier LC3 B, LC3B, MAP1 light chain 3-like protein 2

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, mouse brain tissue, Chloroquine treated HeLa cells, rat brain tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman liver tissue, human gliomas tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inChloroquine treated HeLa cells, Chloroquine treated HepG2 cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy LC3 Ready-To-Use IHC Kit. LC3 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®594 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14600-1-AP targets LC3 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
文献引用应用WB, IHC, IF, IP, CoIP, ELISA
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat, rabbit, monkey, chicken, zebrafish, sheep, goat
免疫原

CatNo: Ag6144

Product name: Recombinant human LC3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-125 aa of BC067797

Sequence: MPSEKTFKQRRTFEQRVEDVRLIREQHPTKIPVIIERYKGEKQLPVLDKTKFLVPDHVNMGELIKIIRRRLQLNANQAFFLLVNGHSMVSVSTPISEVYESEKDEDGFLYMVYASQETFGMKLSV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 microtubule-associated protein 1 light chain 3 beta
别名 MAP1LC3B, ATG8F, Autophagy-related ubiquitin-like modifier LC3 B, LC3B, MAP1 light chain 3-like protein 2
计算分子量 15 kDa
观测分子量 14-18 kDa
GenBank蛋白编号BC067797
基因名称 LC3B
Gene ID (NCBI) 81631
ENSEMBL Gene IDENSG00000140941
RRIDAB_2137737
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9GZQ8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Map1LC3, also known as LC3, is the human homolog of yeast Atg8 and is involved in the formation of autophagosomal vacuoles, called autophagosomes. Three human Map1LC3 isoforms, MAP1LC3A, MAP1LC3B, and MAP1LC3C, undergo post-translational modifications during autophagy. And they differ in their post-translation modifications during autophagy. Map1LC3 also exists in two modified forms, an 18 kDa cytoplasmic form that was originally identified as a subunit of the microtubule-associated protein 1, and a 14-16 kDa form that is associated with the autophagosome membrane. This antibody can cross react with MAP1LC3A, MAP1LC3B, and MAP1LC3C.

实验方案

Product Specific Protocols
FC protocol for LC3 antibody 14600-1-APDownload protocol
IF protocol for LC3 antibody 14600-1-APDownload protocol
IHC protocol for LC3 antibody 14600-1-APDownload protocol
IP protocol for LC3 antibody 14600-1-APDownload protocol
WB protocol for LC3 antibody 14600-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IF

Signal Transduct Target Ther

Nlp promotes autophagy through facilitating the interaction of Rab7 and FYCO1.

Authors - Wenchang Xiao
View Article
humanWB

Signal Transduct Target Ther

Elaiophylin triggers paraptosis and preferentially kills ovarian cancer drug-resistant cells by inducing MAPK hyperactivation

Authors - Guan-Nan Li
View Article
humanWB,IF

Signal Transduct Target Ther

Targeting CRL4 suppresses chemoresistant ovarian cancer growth by inducing mitophagy

Authors - Yang Meng
View Article
mouseIF

Signal Transduct Target Ther

Albumosomes formed by cytoplasmic pre-folding albumin maintain mitochondrial homeostasis and inhibit nonalcoholic fatty liver disease

Authors - Boyuan Ma
View Article
humanWB

Nature

PtdIns(3,5)P(2) is an endogenous ligand of STING in innate immune signalling.

Authors - Jay Xiaojun Tan
View Article
mouseWB

Science

Liver ALKBH5 regulates glucose and lipid homeostasis independently through GCGR and mTORC1 signaling

Authors - Kaixin Ding
View Article
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