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HRP-conjugated Lamin B1 Recombinant antibody

Lamin B1 Uni-rAbTM Recombinant Antibody for WB
Cat No. HRP-80906

产品说明书

CloneNo. 5H10

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB

LMNB1, LMNB, LMN2, Lamin-B1, laminB1

缓冲液配方:  PBS and Azide
PBS and Azide
规格: 

-/ -


经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells
Planning an IHC experiment? We recommend our IHCeasy Lamin B1 Ready-To-Use IHC Kit. Lamin B1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:16000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

HRP-80906 targets Lamin B1 in WB applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB Application Description
经测试反应性 human, mouse, rat
免疫原 Lamin B1 fusion protein Ag3631 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 lamin B1
别名 LMNB1, LMNB, LMN2, Lamin-B1, laminB1
计算分子量 66 kDa
观测分子量 66-70 kDa
GenBank蛋白编号BC012295
基因名称 Lamin B1
Gene ID (NCBI) 4001
ENSEMBL Gene IDENSG00000113368
RRIDAB_3673984
偶联类型 HRP
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP20700
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B1. Expression of uncleavable mutant lamin A or B caused significant delays in the onset of chromatin condensation and nuclear shrinkage during apoptosis (PMID:11953316). This protein is not suitable for samples where the nuclear envelope has been removed.

实验方案

Product Specific Protocols
WB protocol for HRP Lamin B1 antibody HRP-80906Download protocol
Standard Protocols
Click here to view our Standard Protocols
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