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CoraLite® Plus 488-conjugated MEK1/2 Polyclonal antibody

MEK1/2 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-11049

Print datasheet

Synonyms

MAP2K2, MEK2, MAPK/ERK kinase 2, MAP kinase kinase 2, ERK activator kinase 2

验证数据展示

  • IF Staining of HeLa using CL488-11049

    IF Staining of HeLa using CL488-11049

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 MEK1/2 antibody (CL488-11049) at dilution of 1:200.

  • FC experiment of HeLa using CL488-11049

    FC experiment of HeLa using CL488-11049

    1X10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human MEK1/2 (CL488-11049) (red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF/ICC detected inHeLa cells, SH-SY5Y cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy MAP2K2 Ready-To-Use IHC Kit. MAP2K2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 11049-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-11049 targets MEK1/2 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse
Immunogen MEK1/2 fusion protein Ag1481 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name mitogen-activated protein kinase kinase 2
Synonyms MAP2K2, MEK2, MAPK/ERK kinase 2, MAP kinase kinase 2, ERK activator kinase 2
Calculated Molecular Weight 44 kDa
Observed Molecular Weight 44-47 kDa
GenBank Accession NumberBC018645
Gene Symbol MEK2
Gene ID (NCBI) 5605
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP36507
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MEK2(MAPK/ERK kinase 2) is also named as MAP2K2, MKK2, PRKMK2 and belongs to the MAP kinase kinase subfamily. MAPKK is itself dependent on Ser/Thr phosphorylation for activity catalyzed by MAP kinase kinase kinases (RAF or MEKK1). MEK1 and MEK2 are closely related, dual-specificity tyrosine/threonine protein kinases found in the Ras/Raf/MEK/ERK mitogen-activated protein kinase (MAPK) signaling pathway. 11049-1-AP can recognize both the MEK2 and MEK1.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 MEK1/2 antibody CL488-11049Download protocol
FC protocol for CL Plus 488 MEK1/2 antibody CL488-11049Download protocol
Standard Protocols
Click here to view our Standard Protocols