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验证数据展示

  • IF Staining of HEK-293 using CL488-13798

    IF Staining of HEK-293 using CL488-13798

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CoraLite® Plus 488 MFN1 antibody (CL488-13798) at dilution of 1:200.

  • FC experiment of HEK-293 using CL488-13798

    FC experiment of HEK-293 using CL488-13798

    1x10^6 HEK-293 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated MFN1 Polyclonal antibody (CL488-13798)(red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated MFN1 Polyclonal antibody

MFN1 Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-13798

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC, FC (Intra)

别名

Mitofusin-1, mitofusin 1, hfzo2, hfzo1, Fzo homolog

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHEK-293 cells
Positive FC (Intra) detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy MFN1 Ready-To-Use IHC Kit. MFN1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 13798-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-13798 targets MFN1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 MFN1 fusion protein Ag4762 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 mitofusin 1
别名 Mitofusin-1, mitofusin 1, hfzo2, hfzo1, Fzo homolog
计算分子量 741 aa, 84 kDa
观测分子量 86 kDa
GenBank蛋白编号BC040557
基因名称 MFN1
Gene ID (NCBI) 55669
RRIDAB_3672575
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8IWA4
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Mitofusin-1 (MFN1) is a mediator of mitochondrial fusion. This protein and mitofusin 2 are homologs of the Drosophila protein fuzzy onion (Fzo). Mitofusins are large predicted GTPases located in outer mitochondrial membrane. They are essential for outer membrane fusion by interacting with each other to facilitate mitochondrial targeting. The mitofusins are the first known protein mediator of mitochondrial fusion, and mediate developmentally regulated post-meiotic fusion of mitochondria.Mfn1 is required on adjacent mitochondria to mediate fusion via interactions of a heptad repeat region that mediates oligomerization of the protein(PMID:16892085). Mitofusin 1 and mitofusin 2 are ubiquitinated in a PINK1/parkin-dependent manner upon induction of mitophagy(PMID: 20871098).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 MFN1 antibody CL488-13798Download protocol
FC protocol for CL Plus 488 MFN1 antibody CL488-13798Download protocol
Standard Protocols
Click here to view our Standard Protocols