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MMP2 Monoclonal antibody

MMP2 Monoclonal Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, FC (Intra), ELISA and More (1)

Conjugate

Unconjugated

Publications

CloneNo.

2B10D1

Cat no : 66366-1-Ig

Print datasheet

Synonyms

Gelatinase A, EC:3.4.24.24, CLG4A, 72 kDa type IV collagenase, 72 kDa gelatinase

验证数据展示

WB analysis using 66366-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 66366-1-Ig (MMP2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of U-251 using 66366-1-Ig
WB result of MMP2 antibody (66366-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MMP2 transfected U-251 cells.
FC experiment of HeLa using 66366-1-Ig
1x10^6 HeLa cells were intracellularly stained with 0.4 ug MMP2 Monoclonal antibody (66366-1-Ig, Clone:2B10D1) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
IHC staining of human breast cancer using 66366-1-Ig
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66366-1-Ig (MMP2 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with MMP2 antibody included
IHC staining of human breast cancer using 66366-1-Ig
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66366-1-Ig (MMP2 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with MMP2 antibody included

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Cat No. 66366-1-Ig

Size

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经过测试的应用

Positive WB detected in	U-251 cells, human placenta tissue, MCF-7 cells, pig heart tissue, rat heart tissue, U-87 MG cells
Positive IHC detected in	human breast cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive FC (Intra) detected in	HeLa cells, PC-3 cells

Planning an IHC experiment? We recommend our IHCeasy MMP2 Ready-To-Use IHC Kit. MMP2 primary antibody included.

Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunohistochemistry (IHC)	IHC : 1:150-1:600
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WB	See 68 publications below
IHC	See 6 publications below
IF	See 5 publications below

产品信息

66366-1-Ig targets MMP2 in WB, IHC, IF, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications	WB, IHC, FC (Intra), ELISA Application Description
Cited Applications	WB, IHC, IF
Tested Reactivity	human, mouse, rat, pig
Cited Reactivity	human, mouse, rat, pig
Immunogen	MMP2 fusion protein Ag25039 种属同源性预测
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Full Name	matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase)
Synonyms	Gelatinase A, EC:3.4.24.24, CLG4A, 72 kDa type IV collagenase, 72 kDa gelatinase
Calculated Molecular Weight	72 kDa
Observed Molecular Weight	55-74 kDa
GenBank Accession Number	BC002576
Gene Symbol	MMP2
Gene ID (NCBI)	4313
RRID	AB_2881746
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P08253
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

MMP2, also named as CLG4A, Gelatinase Am, TBE-1 and PEX, belongs to the peptidase M10A family. It is ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. MMP2 contributes to myocardial oxidative stress by regulating the activity of GSK3beta. It cleaves GSK3beta in vitro. MMP2 can be cleaved into PEX chain(~60kd).Western blot analysis showed that the 72 kDa and 62 kDa proteinase activities were pro-MMP2 and the active enzyme,respectively(PMID:11112697).

实验方案

Product Specific Protocols
WB protocol for MMP2 antibody 66366-1-Ig	Download protocol
IHC protocol for MMP2 antibody 66366-1-Ig	Download protocol
FC protocol for MMP2 antibody 66366-1-Ig	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
human	IF	Biomaterials Macromechanics and polycaprolactone fiber organization drive macrophage polarization and regulate inflammatory activation of tendon in vitro and in vivo. Authors - Angelina D Schoenenberger View Article
human	WB	Cancer Lett Direct contact between tumor cells and platelets initiates a FAK-dependent F3/TGF-β positive feedback loop that promotes tumor progression and EMT in osteosarcoma Authors - Qianyu Shi View Article
rat	WB,IF	J Neuroinflammation SPOCK2 modulates neuropathic pain by interacting with MT1-MMP to regulate astrocytic MMP-2 activation in rats with chronic constriction injury Authors - Chenglong Wang View Article
rat	WB	Int J Nanomedicine A Novel Liposomal S-Propargyl-Cysteine: A Sustained Release of Hydrogen Sulfide Reducing Myocardial Fibrosis via TGF-β1/Smad Pathway. Authors - Ba Hieu Tran View Article
human	WB	Bioeng Transl Med Multistage targeting and dual inhibiting strategies based on bioengineered tumor matrix microenvironment-mediated protein nanocages for enhancing cancer biotherapy. Authors - Fabiao Hu View Article
human	WB	Aging (Albany NY) Extracorporeal shockwave relieves endothelial injury and dysfunction in steroid-induced osteonecrosis of the femoral head via miR-135b targeting FOXO1: in vitro and in vivo studies. Authors - Xinjie Wu View Article

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MMP2 Monoclonal antibody

MMP2 Monoclonal Antibody for WB, IHC, FC (Intra), ELISA

验证数据展示

WB analysis using 66366-1-Ig

WB analysis of U-251 using 66366-1-Ig

FC experiment of HeLa using 66366-1-Ig

IHC staining of human breast cancer using 66366-1-Ig

IHC staining of human breast cancer using 66366-1-Ig

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

Macromechanics and polycaprolactone fiber organization drive macrophage polarization and regulate inflammatory activation of tendon in vitro and in vivo.

Direct contact between tumor cells and platelets initiates a FAK-dependent F3/TGF-β positive feedback loop that promotes tumor progression and EMT in osteosarcoma

SPOCK2 modulates neuropathic pain by interacting with MT1-MMP to regulate astrocytic MMP-2 activation in rats with chronic constriction injury

A Novel Liposomal S-Propargyl-Cysteine: A Sustained Release of Hydrogen Sulfide Reducing Myocardial Fibrosis via TGF-β1/Smad Pathway.

Multistage targeting and dual inhibiting strategies based on bioengineered tumor matrix microenvironment-mediated protein nanocages for enhancing cancer biotherapy.

Extracorporeal shockwave relieves endothelial injury and dysfunction in steroid-induced osteonecrosis of the femoral head via miR-135b targeting FOXO1: in vitro and in vivo studies.