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验证数据展示

  • WB analysis using 66366-1-Ig (same clone as 66366-1-PBS)

    WB analysis using 66366-1-Ig (same clone as 66366-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66366-1-Ig (MMP2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66366-1-PBS in a different storage buffer formulation.

  • WB analysis of U-251 using 66366-1-Ig (same clone as 66366-1-PBS)

    WB analysis of U-251 using 66366-1-Ig (same clone as 66366-1-PBS)

    WB result of MMP2 antibody (66366-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MMP2 transfected U-251 cells. This data was developed using the same antibody clone with 66366-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 66366-1-Ig (same clone as 66366-1-PBS)

    FC experiment of HeLa using 66366-1-Ig (same clone as 66366-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug MMP2 Monoclonal antibody (66366-1-Ig, Clone:2B10D1) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66366-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66366-1-Ig (same clone as 66366-1-PBS)

    IHC staining of human breast cancer using 66366-1-Ig (same clone as 66366-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66366-1-Ig (MMP2 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66366-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MMP2 antibody included
  • IHC staining of human breast cancer using 66366-1-Ig (same clone as 66366-1-PBS)

    IHC staining of human breast cancer using 66366-1-Ig (same clone as 66366-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66366-1-Ig (MMP2 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66366-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MMP2 antibody included
  • Cytometric bead array standard curve of MP51054-2

    Cytometric bead array standard curve of MP51054-2

    Cytometric bead array standard curve of MP51054-2, MMP2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66366-4-PBS. Detection antibody: 66366-1-PBS. Standard:Ag25039. Range: 0.098-100 ng/mL.

  • Cytometric bead array standard curve of MP51054-3

    Cytometric bead array standard curve of MP51054-3

    Cytometric bead array standard curve of MP51054-3, MMP2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66366-5-PBS. Detection antibody: 66366-1-PBS. Standard:Ag25039. Range: 0.098-100 ng/mL.

  • Featured Product
  • KD/KO验证

MMP2 Monoclonal antibody, PBS Only (Detector)

MMP2 Monoclonal Antibody for WB, IHC, FC (Intra), Cytometric bead array, Indirect ELISA
Cat No. 66366-1-PBS

产品说明书

CloneNo. 2B10D1
引用文献(1)

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig

应用

WB, IHC, FC (Intra), Cytometric bead array, Indirect ELISA

别名

Gelatinase A, EC:3.4.24.24, CLG4A, 72 kDa type IV collagenase, 72 kDa gelatinase

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy MMP2 Ready-To-Use IHC Kit. MMP2 primary antibody included.

发表文章中的应用

IHCSee 1 publications below

产品信息

66366-1-PBS targets MMP2 as part of a matched antibody pair:

MP51054-2: 66366-4-PBS capture and 66366-1-PBS detection (validated in Cytometric bead array)

MP51054-3: 66366-5-PBS capture and 66366-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
文献引用应用IHC
经测试反应性 human, mouse, rat, pig
文献引用反应性human
免疫原 MMP2 fusion protein Ag25039 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase)
别名 Gelatinase A, EC:3.4.24.24, CLG4A, 72 kDa type IV collagenase, 72 kDa gelatinase
计算分子量 72 kDa
观测分子量 55-74 kDa
GenBank蛋白编号BC002576
基因名称 MMP2
Gene ID (NCBI) 4313
RRIDAB_2881746
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP08253
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MMP2, also named as CLG4A, Gelatinase Am, TBE-1 and PEX, belongs to the peptidase M10A family. It is ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. MMP2 contributes to myocardial oxidative stress by regulating the activity of GSK3beta. It cleaves GSK3beta in vitro. MMP2 can be cleaved into PEX chain(~60kd).Western blot analysis showed that the 72 kDa and 62 kDa proteinase activities were pro-MMP2 and the active enzyme,respectively(PMID:11112697).

发表文章

SpeciesApplicationTitle
humanIHC

Int J Mol Sci

Glial-Cell-Line-Derived Neurotrophic Factor Promotes Glioblastoma Cell Migration and Invasion via the SMAD2/3-SERPINE1-Signaling Axis

Authors - Xiaoxiao Guo
View Article