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CoraLite®488-conjugated MMP9 (N-terminal) Polyclonal antibody
MMP9 (N-terminal) Polyclonal Antibody for IF
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF
Conjugate
CoraLite®488 Fluorescent Dye
验证数据展示
经过测试的应用
Positive IF detected in | MCF-7 cells |
推荐稀释比
Application | Dilution |
---|---|
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
产品信息
CL488-10375 targets MMP9 (N-terminal) in IF applications and shows reactivity with human, mouse, rat samples.
Tested Applications | IF |
Tested Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | MMP9 (N-terminal) fusion protein Ag0552 |
Full Name | matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase) |
Synonyms | 92 kDa gelatinase, 92 kDa type IV collagenase, CLG4B, Gelatinase B, GELB, Matrix metalloproteinase 9, MMP 9, MMP9 |
Calculated molecular weight | 707 aa, 78 kDa |
Observed molecular weight | 67 kDa, 92 kDa |
GenBank accession number | BC006093 |
Gene symbol | MMP9 |
Uniprot | P14780 | Gene ID (NCBI) | 4318 |
RRID | AB_2918976 |
Conjugate | CoraLite®488 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 488 nm / 515 nm |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, tissue remodeling, and disease processes, such as arthritis or metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. Matrix metalloproteinase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase) (MMP9, synonyms: GELB, CLG4B) degrades collagens type IV and V. Studies in rhesus monkeys suggest that MMP9 is involved in IL-8-induced mobilization hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. The pro-MMP9 is 92 kDa, and it can be detected a processed form of 68 kDa. This protein can exist as a dimer of 180 kDa (PMID:7492685).
实验方案
Product Specific Protocols | |
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IF protocol for CL488 MMP9 (N-terminal) antibody CL488-10375 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |