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验证数据展示

  • FC experiment of HEK-293 using CL488-66172

    FC experiment of HEK-293 using CL488-66172

    1x10^6 HEK-293 cells were intracellularly stained with 0.8 ug CoraLite®488 Anti-Human MSH6 (CL488-66172, Clone:2E10B2)(red), or 0.8 ug Mouse IgG2a isotype control Mouse McAb (66360-2-Ig, Clone: 11A1B2) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Featured Product
  • KD/KO验证

CoraLite®488-conjugated MSH6 Monoclonal antibody

MSH6 Monoclonal Antibody for FC (Intra)
Cat No. CL488-66172

产品说明书

CloneNo. 2E10B2

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

FC (Intra)

别名

G/T mismatch binding protein, GTBP, GTMBP, hMSH6, HNPCC5, HSAP, MSH6, MutS alpha 160 kDa subunit, mutS homolog 6 (E. coli), p160

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®488
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inHEK-293 cells
Positive FC detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy MSH6 Ready-To-Use IHC Kit. MSH6 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66172-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66172 targets MSH6 in FC (Intra) applications and shows reactivity with human samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human
免疫原 MSH6 fusion protein Ag12645 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 mutS homolog 6 (E. coli)
别名 G/T mismatch binding protein, GTBP, GTMBP, hMSH6, HNPCC5, HSAP, MSH6, MutS alpha 160 kDa subunit, mutS homolog 6 (E. coli), p160
计算分子量 153 kDa
GenBank蛋白编号BC004246
基因名称 MSH6
Gene ID (NCBI) 2956
RRIDAB_2919294
偶联类型 CoraLite®488 Fluorescent Dye
最大激发/发射波长491 nm / 516 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP52701
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MSH6, also named as DNA mismatch repair protein Msh6 or GTMBP, is a 1360 amino acid protein, which contains one PWWP domain and belongs to the DNA mismatch repair MutS family. MSH6 localizes in the nucleus and is a component of the post-replicative DNA mismatch repair system. Msh2 and Msh6 form a protein complex required to repair mismatches generated during DNA replication.

实验方案

Product Specific Protocols
FC protocol for CL488 MSH6 antibody CL488-66172Download protocol
Standard Protocols
Click here to view our Standard Protocols