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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 647-conjugated MSH6 Monoclonal antibody

MSH6 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

2E10B2

Cat no : CL647-66172

Print datasheet

Synonyms

hMSH6, GTMBP, GTBP, G/T mismatch-binding protein, G/T mismatch binding protein

验证数据展示

  • FC experiment of HEK-293 using CL647-66172

    FC experiment of HEK-293 using CL647-66172

    1X10^6 HEK-293 cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human MSH6 (CL647-66172, Clone:2E10B2) (red), or 0.2 ug isotype control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive FC (Intra) detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy MSH6 Ready-To-Use IHC Kit. MSH6 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66172-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-66172 targets MSH6 in FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human, mouse
Immunogen MSH6 fusion protein Ag12645 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name mutS homolog 6 (E. coli)
Synonyms hMSH6, GTMBP, GTBP, G/T mismatch-binding protein, G/T mismatch binding protein
Calculated Molecular Weight 153 kDa
Observed Molecular Weight 160 kDa
GenBank Accession NumberBC004246
Gene Symbol MSH6
Gene ID (NCBI) 2956
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP52701
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MSH6, also named as DNA mismatch repair protein Msh6 or GTMBP, is a 1360 amino acid protein, which contains one PWWP domain and belongs to the DNA mismatch repair MutS family. MSH6 localizes in the nucleus and is a component of the post-replicative DNA mismatch repair system. Msh2 and Msh6 form a protein complex required to repair mismatches generated during DNA replication.

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 MSH6 antibody CL647-66172Download protocol
Standard Protocols
Click here to view our Standard Protocols