验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, human skeletal muscle tissue, mouse liver tissue |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human ovary cancer tissue, human kidney tissue, human spleen tissue, human skin tissue, rat stomach tissue, human placenta tissue, human heart tissue, rat heart tissue, mouse stomach tissue, human ovary tissue, human brain tissue, human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 10 publications below |
产品信息
15589-1-AP targets NDUFB10 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag7922 Product name: Recombinant human NDUFB10 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-172 aa of BC005829 Sequence: MPDSWDKDVYPEPPRRTPVQPNPIVYMMKAFDLIVDRPVTLVREFIERQHAKNRYYYYHRQYRRVPDITECKEEDIMCMYEAEMQWKRDYKVDQEIINIMQDRLKACQQREGQNYQQNCIKEVEQFTQVAKAYQDRYQDLGAYSSARKCLAKQRQRMLQERKAAKEAAAATS 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 10, 22kDa |
| 别名 | CI PDSW, CI-PDSW, Complex I PDSW, Complex I-PDSW, NADH-ubiquinone oxidoreductase PDSW subunit |
| 计算分子量 | 22 kDa |
| 观测分子量 | 22 kDa |
| GenBank蛋白编号 | BC005829 |
| 基因名称 | NDUFB10 |
| Gene ID (NCBI) | 4716 |
| RRID | AB_2150790 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O96000 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NDUFB10(NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10) is also named as PDSW and belongs to the complex I NDUFB10 subunit family. It is an accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. The Complex I functions in the transfer of electrons from NADH to the respiratory chain.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for NDUFB10 antibody 15589-1-AP | Download protocol |
| IP protocol for NDUFB10 antibody 15589-1-AP | Download protocol |
| WB protocol for NDUFB10 antibody 15589-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Stem Cell LIN28 Regulates Stem Cell Metabolism and Conversion to Primed Pluripotency. | ||
Nat Cell Biol LIN28 phosphorylation by MAPK/ERK couples signalling to the post-transcriptional control of pluripotency. | ||
Mol Cell Identification of TMEM126A as OXA1L-interacting protein reveals cotranslational quality control in mitochondria | ||
Cell Rep A Role for Mitochondrial Translation in Promotion of Viability in K-Ras Mutant Cells.
| ||
Chin J Cancer Res Molecular subtypes and prognostic signature rooted in disulfidptosis highlight tumor microenvironment in lung adenocarcinoma. |
