验证数据展示
经过测试的应用
| Positive IF-P detected in | mouse brain tissue |
Planning an IHC experiment? We recommend our IHCeasy NF-L Ready-To-Use IHC Kit. NF-L primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 60189-1-Ig
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-60189 targets NF-L in IF-P applications and shows reactivity with human, mouse, rat, pig samples.
| 经测试应用 | IF-P Application Description |
| 经测试反应性 | human, mouse, rat, pig |
| 免疫原 | NF-L fusion protein Ag15178 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | neurofilament, light polypeptide |
| 别名 | 68 kDa neurofilament protein, CMT1F, CMT2E, FLJ53642, NEFL, neurofilament, NF, NF L, NF68, NFL, NF-L |
| 计算分子量 | 543 aa, 62 kDa |
| GenBank蛋白编号 | BC039237 |
| 基因名称 | NF-L |
| Gene ID (NCBI) | 4747 |
| RRID | AB_2883115 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P07196 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NEFL, also named as NF68 and NF-L, belongs to the intermediate filament family. Neurofilaments are the 10 nm intermediate filaments found specifically in neurons. They are a major component of the cell's cytoskeleton, and provide support for normal axonal radial growth. Neurofilaments usually contain three intermediate filament proteins: L, M, and H, which are involved in the maintenance of neuronal caliber. This antibody is conjugated with CL488, Ex/Em 488 nm/515 nm.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 NF-L antibody CL488-60189 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |