CoraLite® Plus 488-conjugated NF-L Monoclonal antibody
NF-L Monoclonal Antibody for IF-P
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat, pig
Applications
IF-P
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
5C12G4
验证数据展示
经过测试的应用
Positive IF-P detected in | mouse brain tissue |
Planning an IHC experiment? We recommend our IHCeasy NF-L Ready-To-Use IHC Kit. NF-L primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 60189-1-Ig
推荐稀释比
Application | Dilution |
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Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL488-60189 targets NF-L in IF-P applications and shows reactivity with human, mouse, rat, pig samples.
Tested Applications | IF-P Application Description |
Tested Reactivity | human, mouse, rat, pig |
Immunogen | NF-L fusion protein Ag15178 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | neurofilament, light polypeptide |
Synonyms | 68 kDa neurofilament protein, CMT1F, CMT2E, FLJ53642, NEFL, neurofilament, NF, NF L, NF68, NFL, NF-L |
Calculated Molecular Weight | 543 aa, 62 kDa |
GenBank Accession Number | BC039237 |
Gene Symbol | NF-L |
Gene ID (NCBI) | 4747 |
RRID | AB_2883115 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein G purification |
UNIPROT ID | P07196 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NEFL, also named as NF68 and NF-L, belongs to the intermediate filament family. Neurofilaments are the 10 nm intermediate filaments found specifically in neurons. They are a major component of the cell's cytoskeleton, and provide support for normal axonal radial growth. Neurofilaments usually contain three intermediate filament proteins: L, M, and H, which are involved in the maintenance of neuronal caliber. This antibody is conjugated with CL488, Ex/Em 488 nm/515 nm.
实验方案
Product Specific Protocols | |
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IF protocol for CL Plus 488 NF-L antibody CL488-60189 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |