CoraLite®594-conjugated NF-L Monoclonal antibody
NF-L Monoclonal Antibody for IF-P, FC (Intra)
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat, pig
Applications
IF-P, FC (Intra)
Conjugate
CoraLite®594 Fluorescent Dye
CloneNo.
5C12G4
验证数据展示
经过测试的应用
| Positive IF-P detected in | mouse brain tissue |
| Positive FC (Intra) detected in | SH-SY5Y cells |
Planning an IHC experiment? We recommend our IHCeasy NF-L Ready-To-Use IHC Kit. NF-L primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 60189-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL594-60189 targets NF-L in IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat, pig samples.
| Tested Applications | IF-P, FC (Intra) Application Description |
| Tested Reactivity | human, mouse, rat, pig |
| Immunogen | NF-L fusion protein Ag15178 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | neurofilament, light polypeptide |
| Synonyms | NEFL, NFL, NF68, neurofilament, 5C12G4 |
| Calculated Molecular Weight | 543 aa, 62 kDa |
| Observed Molecular Weight | 65 kDa |
| GenBank Accession Number | BC039237 |
| Gene Symbol | NF-L |
| Gene ID (NCBI) | 4747 |
| Conjugate | CoraLite®594 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 588 nm / 604 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P07196 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NEFL, also named as NF68 and NF-L, belongs to the intermediate filament family. Neurofilaments are the 10 nm intermediate filaments found specifically in neurons. They are a major component of the cell's cytoskeleton, and provide support for normal axonal radial growth. Neurofilaments usually contain three intermediate filament proteins: L, M, and H, which are involved in the maintenance of neuronal caliber. The names given to the three major neurofilament subunits are based upon the apparent molecular weight of the mammalian subunits on SDS-PAGE: NF-L, 65-68 kDa; NF-M,145-160 kDa and NF-H, 200-220 kDa.This antibody is specific to NEFL.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 NF-L antibody CL594-60189 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
