验证数据展示
经过测试的应用
Positive WB detected in | human placenta tissue, fetal human brain tissue, human plasma, 37°C incubated human placenta tissue, pig brain tissue, HeLa cells, rabbit heart tissue, MDA-MB-231 cells, A549 cells, pig heart tissue |
Positive IF/ICC detected in | SH-SY5Y cells, human embronic stem cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:1000-1:6000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
60067-1-Ig targets Neuropilin 1 in WB, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, pig, rabbit samples.
经测试应用 | WB, IF/ICC, ELISA Application Description |
文献引用应用 | WB, IF, IP, CoIP |
经测试反应性 | human, pig, rabbit |
文献引用反应性 | human, mouse, rabbit, xenopus |
免疫原 | Neuropilin 1 fusion protein Ag0931 种属同源性预测 |
宿主/亚型 | Mouse / IgG1 |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | neuropilin 1 |
别名 | NRP1, Neuropilin-1, CD304, BDCA4, 2H3F6 |
计算分子量 | 103 kDa |
观测分子量 | 130 kDa |
GenBank蛋白编号 | BC007737 |
基因名称 | Neuropilin 1 |
Gene ID (NCBI) | 8829 |
RRID | AB_2150840 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | O14786 |
储存缓冲液 | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Neuropilin-1 (NRP1) is a 130-140 kDa transmembrane glycoprotein expressed by endothelial, dendritic, and regulatory T cells, as well as several other normal cell types and malignant tumor cells. NRP1 was first identified as a semaphorin (SEMA) receptor, involved in axonal guidance in embryonic development. NRP1 was also shown to act as a receptor for vascular endothelial growth factor (VEGF) and a promoter of angiogenesis through its interaction with VEGF-A165 (and other VEGFs) and the receptor tyrosine kinase (RTK) VEGF-R2. NRP1 plays versatile roles in angiogenesis, axon guidance, cell survival, migration, and invasion.
实验方案
Product Specific Protocols | |
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WB protocol for Neuropilin 1 antibody 60067-1-Ig | Download protocol |
IF protocol for Neuropilin 1 antibody 60067-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Elife Receptor-specific interactome as a hub for rapid cue-induced selective translation in axons. | ||
Acta Biomater Semaphorin-3A, Neuropilin-1 and Plexin-A1 in Prosthetic-Particle Induced Bone Loss. | ||
J Pathol Perivascular Neuropilin-1 expression is an independent marker of improved survival in renal cell carcinoma. | ||
Am J Cancer Res CMTM6 promotes cell proliferation and invasion in oral squamous cell carcinoma by interacting with NRP1. | ||
Pharm Res Dual Receptor Targeting Cell Penetrating Peptide Modified Liposome for Glioma and Breast Cancer Postoperative Recurrence Therapy. | ||
Exp Eye Res Expression of axon guidance ligands and their receptors in the cornea and trigeminal ganglia and their recovery after corneal epithelium injury. |