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Neuropilin 1 Monoclonal antibody, PBS Only

Neuropilin 1 Monoclonal Antibody for WB, IF, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, pig, rabbit

Applications

WB, IF, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2H3F6

Cat No : 60067-1-PBS

Print datasheet

Synonyms

BDCA4, CD304, neuropilin 1, NRP, NRP1, VEGF165R

验证数据展示

  • WB analysis using 60067-1-Ig (same clone as 60067-1-PBS)

    WB analysis using 60067-1-Ig (same clone as 60067-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60067-1-Ig (Neuropilin 1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60067-1-PBS in a different storage buffer formulation.

  • IF Staining of human embronic stem cells using 60067-1-Ig (same clone as 60067-1-PBS)

    IF Staining of human embronic stem cells using 60067-1-Ig (same clone as 60067-1-PBS)

    Confocal immunofluorescent analysis of human embronic stem cells with 60067-1-Ig at dilution of 1:100. DAPI was used to stain the cell nuclei (blue) at a concentration of 1ug/ml. This photo is the merge of PE and DAPI. (40X). This data was developed using the same antibody clone with 60067-1-PBS in a different storage buffer formulation.

  • IF Staining of SH-SY5Y using 60067-1-Ig (same clone as 60067-1-PBS)

    IF Staining of SH-SY5Y using 60067-1-Ig (same clone as 60067-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol ) fixed SH-SY5Y cells using 60067-1-Ig(Neuropilin 1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60067-1-PBS in a different storage buffer formulation.

产品信息

60067-1-PBS targets Neuropilin 1 in WB, IF, Indirect ELISA applications and shows reactivity with human, pig, rabbit samples.

Tested Applications WB, IF, Indirect ELISA Application Description
Tested Reactivity human, pig, rabbit
Immunogen Neuropilin 1 fusion protein Ag0931 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name neuropilin 1
Synonyms BDCA4, CD304, neuropilin 1, NRP, NRP1, VEGF165R
Calculated Molecular Weight 103 kDa
Observed Molecular Weight 130 kDa
GenBank Accession NumberBC007737
Gene Symbol NRP1
Gene ID (NCBI) 8829
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO14786
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

Neuropilin-1 (NRP1) is a 130-140 kDa transmembrane glycoprotein expressed by endothelial, dendritic, and regulatory T cells, as well as several other normal cell types and malignant tumor cells. NRP1 was first identified as a semaphorin (SEMA) receptor, involved in axonal guidance in embryonic development. NRP1 was also shown to act as a receptor for vascular endothelial growth factor (VEGF) and a promoter of angiogenesis through its interaction with VEGF-A165 (and other VEGFs) and the receptor tyrosine kinase (RTK) VEGF-R2. NRP1 plays versatile roles in angiogenesis, axon guidance, cell survival, migration, and invasion.