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  • Featured Product
  • KD/KO Validated

Neuropilin 1 Monoclonal antibody, PBS Only (Capture)

Neuropilin 1 Monoclonal Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, pig, rabbit

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2H3F6

Cat no : 60067-1-PBS

Print datasheet

Synonyms

NRP1, Neuropilin-1, CD304, BDCA4, 2H3F6

验证数据展示

  • WB analysis using 60067-1-Ig (same clone as 60067-1-PBS)

    WB analysis using 60067-1-Ig (same clone as 60067-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60067-1-Ig (Neuropilin 1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60067-1-PBS in a different storage buffer formulation.

  • IF Staining of human embronic stem cells using 60067-1-Ig (same clone as 60067-1-PBS)

    IF Staining of human embronic stem cells using 60067-1-Ig (same clone as 60067-1-PBS)

    Confocal immunofluorescent analysis of human embronic stem cells with 60067-1-Ig at dilution of 1:100. DAPI was used to stain the cell nuclei (blue) at a concentration of 1ug/ml. This photo is the merge of PE and DAPI. (40X). This data was developed using the same antibody clone with 60067-1-PBS in a different storage buffer formulation.

  • IF Staining of SH-SY5Y using 60067-1-Ig (same clone as 60067-1-PBS)

    IF Staining of SH-SY5Y using 60067-1-Ig (same clone as 60067-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol ) fixed SH-SY5Y cells using 60067-1-Ig(Neuropilin 1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60067-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50479-1

    Cytometric bead array standard curve of MP50479-1

    Cytometric bead array standard curve of MP50479-1, NRP1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60067-1-PBS. Detection antibody: 60067-2-PBS. Standard:Ag0931. Range: 0.391-100 ng/mL.

产品信息

60067-1-PBS targets Neuropilin 1 as part of a matched antibody pair:

MP50479-1: 60067-1-PBS capture and 60067-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human, pig, rabbit
Immunogen Neuropilin 1 fusion protein Ag0931 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name neuropilin 1
Synonyms NRP1, Neuropilin-1, CD304, BDCA4, 2H3F6
Calculated Molecular Weight 103 kDa
Observed Molecular Weight 130 kDa
GenBank Accession NumberBC007737
Gene Symbol Neuropilin 1
Gene ID (NCBI) 8829
RRIDAB_2150840
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO14786
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Neuropilin-1 (NRP1) is a 130-140 kDa transmembrane glycoprotein expressed by endothelial, dendritic, and regulatory T cells, as well as several other normal cell types and malignant tumor cells. NRP1 was first identified as a semaphorin (SEMA) receptor, involved in axonal guidance in embryonic development. NRP1 was also shown to act as a receptor for vascular endothelial growth factor (VEGF) and a promoter of angiogenesis through its interaction with VEGF-A165 (and other VEGFs) and the receptor tyrosine kinase (RTK) VEGF-R2. NRP1 plays versatile roles in angiogenesis, axon guidance, cell survival, migration, and invasion.