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验证数据展示

  • FC experiment of HEK-293 using CL647-69011

    FC experiment of HEK-293 using CL647-69011

    1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug CoraLite® Plus 647 Anti-Human BMP-7 (CL647-69011, Clone:3E9G7) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 647-conjugated BMP-7 Monoclonal antibody

BMP-7 Monoclonal Antibody for FC (Intra)
Cat No. CL647-69011

产品说明书

CloneNo. 3E9G7

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

FC (Intra)

别名

BMP7, OP 1, Eptotermin alfa, Bone morphogenetic protein 7, BMP 7

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 647
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inHEK-293 cells
For other applications, we recommend the unconjugated version of this antibody, 69011-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-69011 targets BMP-7 in FC (Intra) applications and shows reactivity with human samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human
免疫原 BMP-7 fusion protein HZ-1229 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 bone morphogenetic protein 7
别名 BMP7, OP 1, Eptotermin alfa, Bone morphogenetic protein 7, BMP 7
基因名称 BMP7
Gene ID (NCBI) 655
RRIDAB_2920323
偶联类型 CoraLite® Plus 647 Fluorescent Dye
最大激发/发射波长654 nm / 674 nm
形式 Liquid
纯化方式Protein G purification
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The bone morphogenetic proteins (BMPs) are a family of secreted signaling molecules that can induce ectopic bone growth. Many BMPs are part of the transforming growth factor-beta (TGFB) superfamily. BMPs were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site.

BMP7, also known as osteogenic protein-1 or OP-1, plays a key role in the transformation of mesenchymal cells into bone and cartilage. BMP7 may be involved in bone homeostasis (PMID: 15621726). It is expressed in the brain, kidneys and bladder. BMP7 is also present in cancers, including breast, prostate, and colon cancers, in which it is implicated in regulating cancer cell proliferation (PMID: 16419056, PMID: 15531927). Overexpression of BMP7 mRNA in colorectal cancer patients was significantly associated with poor prognosis and low overall survival (PMID: 18259822). Recent studies suggest that high-expression level of BMP7 serves as a biomarker for poor prognosis for HCC (PMID: 23179403).

This antibody is used to Block/Neutralize BMP-7 protein.

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 BMP-7 antibody CL647-69011Download protocol
Standard Protocols
Click here to view our Standard Protocols