CoraLite® Plus 647-conjugated BMP-7 Monoclonal antibody
BMP-7 Monoclonal Antibody for FC (Intra)
Host / Isotype
Mouse / IgG1
Reactivity
human
Applications
FC (Intra)
Conjugate
CoraLite® Plus 647 Fluorescent Dye
CloneNo.
3E9G7
验证数据展示
经过测试的应用
Positive FC (Intra) detected in | HEK-293 cells |
For other applications, we recommend the unconjugated version of this antibody, 69011-1-Ig
推荐稀释比
Application | Dilution |
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Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL647-69011 targets BMP-7 in FC (Intra) applications and shows reactivity with human samples.
Tested Applications | FC (Intra) Application Description |
Tested Reactivity | human |
Immunogen | BMP-7 fusion protein HZ-1229 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | bone morphogenetic protein 7 |
Synonyms | BMP7, OP 1, Eptotermin alfa, Bone morphogenetic protein 7, BMP 7 |
Gene Symbol | BMP7 |
Gene ID (NCBI) | 655 |
RRID | AB_2920323 |
Conjugate | CoraLite® Plus 647 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 654 nm / 674 nm |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The bone morphogenetic proteins (BMPs) are a family of secreted signaling molecules that can induce ectopic bone growth. Many BMPs are part of the transforming growth factor-beta (TGFB) superfamily. BMPs were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site.
BMP7, also known as osteogenic protein-1 or OP-1, plays a key role in the transformation of mesenchymal cells into bone and cartilage. BMP7 may be involved in bone homeostasis (PMID: 15621726). It is expressed in the brain, kidneys and bladder. BMP7 is also present in cancers, including breast, prostate, and colon cancers, in which it is implicated in regulating cancer cell proliferation (PMID: 16419056, PMID: 15531927). Overexpression of BMP7 mRNA in colorectal cancer patients was significantly associated with poor prognosis and low overall survival (PMID: 18259822). Recent studies suggest that high-expression level of BMP7 serves as a biomarker for poor prognosis for HCC (PMID: 23179403).
This antibody is used to Block/Neutralize BMP-7 protein.
BMP7, also known as osteogenic protein-1 or OP-1, plays a key role in the transformation of mesenchymal cells into bone and cartilage. BMP7 may be involved in bone homeostasis (PMID: 15621726). It is expressed in the brain, kidneys and bladder. BMP7 is also present in cancers, including breast, prostate, and colon cancers, in which it is implicated in regulating cancer cell proliferation (PMID: 16419056, PMID: 15531927). Overexpression of BMP7 mRNA in colorectal cancer patients was significantly associated with poor prognosis and low overall survival (PMID: 18259822). Recent studies suggest that high-expression level of BMP7 serves as a biomarker for poor prognosis for HCC (PMID: 23179403).
This antibody is used to Block/Neutralize BMP-7 protein.
实验方案
Product Specific Protocols | |
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FC protocol for CL Plus 647 BMP-7 antibody CL647-69011 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |