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NeutraKine®FGF basic Monoclonal antibody, PBS Only (Detector)

NeutraKine®FGF basic Monoclonal Antibody for Cytometric bead array, Indirect ELISA, Neutralization

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

Cytometric bead array, Indirect ELISA, Neutralization

Conjugate

Unconjugated

CloneNo.

3C9C3

Cat no : 69024-1-PBS

Print datasheet

Synonyms

验证数据展示

  • Neutralization experiment of NeutraKine®FGF basic using 69024-1-Ig (same clone as 69024-1-PBS)

    Neutralization experiment of NeutraKine®FGF basic using 69024-1-Ig (same clone as 69024-1-PBS)

    Recombinant human FGF Basic (Cat.NO. HZ-1285) stimulates the proliferation of BALB/3T3 cells (mouse fibroblast cell) at low FBS condition in a dose-dependent manner (blue curve, refer to bottom X-left Y axis). The activity of human FGF Basic (0.1 ng/mL HZ-1285) is neutralized by mouse anti-human FGF Basic monoclonal antibody 69024-1-Ig at serial dose (red curve, refer to top X-right Y axis). The ND50 is typically 1-10 μg/mL. Mouse IgG1 Isotype Control monoclonal antibody 66360-1-Ig was served as control (green curve, refer to top X-right Y axis). This data was developed using the same antibody clone with 69024-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50262-2

    Cytometric bead array standard curve of MP50262-2

    Cytometric bead array standard curve of MP50262-2, FGF2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68870-2-PBS. Detection antibody: 69024-1-PBS. Standard:HZ-1285. Range: 0.391-100 ng/mL.

Planning an IHC experiment? We recommend our IHCeasy FGF2 Ready-To-Use IHC Kit. FGF2 primary antibody included.

产品信息

69024-1-PBS targets NeutraKine®FGF basic as part of a matched antibody pair:

MP50262-2: 68870-2-PBS capture and 69024-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications Cytometric bead array, Indirect ELISA, Neutralization Application Description
Tested Reactivity human
Immunogen human HumankineFGF basic protein HZ-1285 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name fibroblast growth factor 2 (basic)
Synonyms
Gene Symbol FGF2
Gene ID (NCBI) 2247
RRIDAB_2918844
Conjugate Unconjugated
Form Lyophilized Powder
Purification MethodProtein G purification
Storage Buffer PBS Only
Endotoxin<0.1 EU/μg
ReconstitutionThis product was lyophilized from a 0.2 μm filtered solution in PBS. Reconstitute at 1.0 mg/mL in sterile H2O before use.
Stability and StorageLyophilized antibodies are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.

背景介绍

Fibroblast growth factor-2 (FGF-2), also referred to as basic FGF, belongs to a family of growth factors that stimulate proliferation of cells of mesenchymal, epithelial and neuroectodermal origin. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in at least five different isoforms with distinct properties.

This antibody is used to neutralize the bioactivity of FGFbasic-TS.