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NeutraKine®FGF basic Monoclonal antibody

NeutraKine®FGF basic Monoclonal Antibody for ELISA, Neutralization

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (1)

Applications

ELISA, Neutralization and More (1)

Conjugate

Unconjugated

CloneNo.

3C9C3

Cat no : 69024-1-Ig

Print datasheet

Synonyms

Basic fibroblast growth factor, BFGF, FGF basic, FGF2, FGFB, FGFbasic, HBGF 2

验证数据展示

  • Neutralization experiment of NeutraKine®FGF basic using 69024-1-Ig

    Neutralization experiment of NeutraKine®FGF basic using 69024-1-Ig

    Recombinant human FGF Basic (Cat.NO. HZ-1285) stimulates the proliferation of BALB/3T3 cells (mouse fibroblast cell) at low FBS condition in a dose-dependent manner (blue curve, refer to bottom X-left Y axis). The activity of human FGF Basic (0.1 ng/mL HZ-1285) is neutralized by mouse anti-human FGF Basic monoclonal antibody 69024-1-Ig at serial dose (red curve, refer to top X-right Y axis). The ND50 is typically 1-10 μg/mL. Mouse IgG1 Isotype Control monoclonal antibody 66360-1-Ig was served as control (green curve, refer to top X-right Y axis).

Planning an IHC experiment? We recommend our IHCeasy FGF2 Ready-To-Use IHC Kit. FGF2 primary antibody included.
Neutralization

产品信息

69024-1-Ig targets NeutraKine®FGF basic in ELISA, Neutralization, Cell treatment applications and shows reactivity with Human samples.

Tested Applications ELISA, Neutralization Application Description
Cited ApplicationsCell treatment
Tested Reactivity Human
Cited Reactivityhuman
Immunogen human HumankineFGF basic protein HZ-1285 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name fibroblast growth factor 2 (basic)
Synonyms Basic fibroblast growth factor, BFGF, FGF basic, FGF2, FGFB, FGFbasic, HBGF 2
Gene Symbol FGF2
Gene ID (NCBI) 2247
RRIDAB_2918844
Conjugate Unconjugated
Form Lyophilized Powder
Purification MethodProtein G purification
Storage Buffer Sterile PBS.
Endotoxin<0.1 EU/μg
ReconstitutionThis product was lyophilized from a 0.2 μm filtered solution in PBS. Reconstitute at 1.0 mg/mL in sterile H2O before use.
Stability and StorageLyophilized antibodies are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.

背景介绍

Fibroblast growth factor-2 (FGF-2), also referred to as basic FGF, belongs to a family of growth factors that stimulate proliferation of cells of mesenchymal, epithelial and neuroectodermal origin. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in at least five different isoforms with distinct properties.

This antibody is used to neutralize the bioactivity of FGFbasic-TS.

发表文章

SpeciesApplicationTitle
humanCell treatment

Int Immunopharmacol

Cancer-associated fibroblasts promote gastric cancer cell proliferation by paracrine FGF2-driven ribosome biogenesis

Authors - Dandan Li
View Article