验证数据展示

WB analysis using 60283-2-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 60283-2-Ig (P53 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
IHC staining of human colon cancer using 60283-2-Ig
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60283-2-Ig (P53 antibody) at dilution of 1:3200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with P53 antibody included
WB analysis of HSC-T6 using 60283-2-Ig
HSC-T6 cells were subjected to SDS PAGE followed by western blot with 60283-2-Ig (P53 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
WB analysis of ROS1728 using 60283-2-Ig
ROS1728 cells were subjected to SDS PAGE followed by western blot with 60283-2-Ig (P53 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
WB analysis of rat thymus using 60283-2-Ig
rat thymus tissue were subjected to SDS PAGE followed by western blot with 60283-2-Ig (P53 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
IHC staining of human ovary cancer using 60283-2-Ig
Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 60283-2-Ig (P53 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with P53 antibody included
IF Staining of A431 using 60283-2-Ig
Immunofluorescent analysis of (4% PFA) fixed A431 cells using P53 antibody (60283-2-Ig, Clone: 6C4B6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red).

Featured Product
KD/KO Validated

P53 Monoclonal antibody

P53 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 60283-2-Ig

Print datasheet

CloneNo. 6C4B6

Publications(272)

Host / Isotype

Mouse / IgG2b

Reactivity

human, rat and More (4)

Applications

WB, IHC, IF/ICC, ELISA and More (3)

Synonyms

TP53, Tumour Protein p53, Tumor suppressor p53, tumor protein p53, Antigen NY-CO-13

Formulation: PBS and Azide

PBS and Azide

PBS Only

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite®594

Size:

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概述

经过测试的应用
产品信息
发表文章 (272)
实验方案

Print datasheet

MSDS

经过测试的应用

Positive WB detected in	A431 cells, COLO 320 cells, HSC-T6 cells, ROS1728 cells, rat thymus tissue, HaCaT cells, HT-29 cells, HEK-293 cells
Positive IHC detected in	human colon cancer tissue, human breast cancer tissue, human gliomas tissue, human lung cancer tissue, human ovary cancer tissue, human ovary tumor tissue, human stomach cancer tissue, human tonsillitis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	A431 cells

Planning an IHC experiment? We recommend our IHCeasy P53 Ready-To-Use IHC Kit. P53 primary antibody included.

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunohistochemistry (IHC)	IHC : 1:1600-1:6400
Immunofluorescence (IF)/ICC	IF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 5 publications below
WB	See 245 publications below
IHC	See 31 publications below
IF	See 35 publications below
IP	See 11 publications below
CoIP	See 6 publications below

产品信息

60283-2-Ig targets P53 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, rat samples.

Tested Applications	WB, IHC, IF/ICC, ELISA Application Description
Cited Applications	WB, IHC, IF, IP, CoIP
Tested Reactivity	human, rat
Cited Reactivity	human, rat, pig, rabbit, canine, zebrafish
Immunogen	P53 fusion protein Ag0698 种属同源性预测
Host / Isotype	Mouse / IgG2b
Class	Monoclonal
Type	Antibody
Full Name	tumor protein p53
Synonyms	TP53, Tumour Protein p53, Tumor suppressor p53, tumor protein p53, Antigen NY-CO-13
Calculated Molecular Weight	44 kDa
Observed Molecular Weight	53 kDa
GenBank Accession Number	BC003596
Gene Symbol	P53
Gene ID (NCBI)	7157
RRID	AB_2881401
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P04637
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

1. What is p53?

P53 is a tumor suppressor gene that plays a role in maintaining genomic stability and controlling apoptosis. During the cell cycle, it can arrest cells at the G1/S checkpoint and activate DNA repair mechanisms. It is the most mutated gene in cancer. In unstressed cells, p53 usually exists at low levels in an inactive form, being bound to Mdm2.

2. FAQs and p53

a. I fail to detect p53 by western blotting

Basal levels of wild-type p53 in untreated cells can be low. Try to load more cell lysate and use a positive control - a lysate of cells treated with DNA-damaging agents should increase p53 levels.

b. I fail to detect p53 in some cell lines by western blotting

Various p53 mutations are present in cancer cell types. If mutations cause truncations/deletions some monoclonal antibodies may no longer recognize mutated p53. You have more chances of detecting various p53 mutants with our polyclonal antibody.

c. I can detect more than one band ~50 kDa size / different cell lines give bands at slightly different size

p53 is a subject of post-translational modifications (http://p53.free.fr/p53_info/p53_modifications.html) and more than one isoform may be expressed (http://p53.free.fr/p53_info/p53_isoforms.html). Also, it is possible that your cell line of interest expresses one allele with mutated p53 with altered molecular weight.

实验方案

Product Specific Protocols
WB protocol for P53 antibody 60283-2-Ig	Download protocol
IHC protocol for P53 antibody 60283-2-Ig	Download protocol
IF protocol for P53 antibody 60283-2-Ig	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
mouse	WB	Bioact Mater Chemo-immunotherapy by dual-enzyme responsive peptide self-assembling abolish melanoma Authors - Yuhan Wang View Article
mouse	WB	Nat Commun Circular RNA encoded MET variant promotes glioblastoma tumorigenesis Authors - Jian Zhong View Article
mouse	WB	Redox Biol Deficiency of S100 calcium binding protein A9 attenuates vascular dysfunction in aged mice Authors - Boying Zhao View Article
mouse	IHC	Aging (Albany NY) Whole-transcriptome sequencing analysis reveal mechanisms of Yiqi Huoxue Yangyin (YHY) decoction in ameliorating D-gal-induced cardiac aging Authors - Xue Wang View Article
	WB	J Adv Res TRPM2-mediated feed-forward loop promotes chondrocyte damage in osteoarthritis via calcium-cGAS-STING-NF-κB pathway Authors - Kai Sun View Article
human	WB	Clin Transl Med p53 inhibits OTUD5 transcription to promote GPX4 degradation and induce ferroptosis in gastric cancer Authors - Junjing Zhang View Article

验证数据展示

WB analysis using 60283-2-Ig

IHC staining of human colon cancer using 60283-2-Ig

WB analysis of HSC-T6 using 60283-2-Ig

WB analysis of ROS1728 using 60283-2-Ig

WB analysis of rat thymus using 60283-2-Ig

IHC staining of human ovary cancer using 60283-2-Ig

IF Staining of A431 using 60283-2-Ig

P53 Monoclonal antibody

P53 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

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经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

Chemo-immunotherapy by dual-enzyme responsive peptide self-assembling abolish melanoma

Circular RNA encoded MET variant promotes glioblastoma tumorigenesis

Deficiency of S100 calcium binding protein A9 attenuates vascular dysfunction in aged mice

Whole-transcriptome sequencing analysis reveal mechanisms of Yiqi Huoxue Yangyin (YHY) decoction in ameliorating D-gal-induced cardiac aging

TRPM2-mediated feed-forward loop promotes chondrocyte damage in osteoarthritis via calcium-cGAS-STING-NF-κB pathway

p53 inhibits OTUD5 transcription to promote GPX4 degradation and induce ferroptosis in gastric cancer