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P53 Monoclonal antibody

P53 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 60283-2-Ig

产品说明书

CloneNo. 6C4B6

宿主/亚型

Mouse / IgG2b

种属反应性

human, rat and More (4)

应用

WB, IHC, IF/ICC, ELISA and More (3)

TP53, Tumour Protein p53, Tumor suppressor p53, tumor protein p53, Antigen NY-CO-13

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inA431 cells, COLO 320 cells, HSC-T6 cells, ROS1728 cells, rat thymus tissue, HaCaT cells, HT-29 cells, HEK-293 cells
Positive IHC detected inhuman colon cancer tissue, human breast cancer tissue, human gliomas tissue, human lung cancer tissue, human ovary cancer tissue, human ovary tumor tissue, human stomach cancer tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy P53 Ready-To-Use IHC Kit. P53 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1600-1:6400
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60283-2-Ig targets P53 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IHC, IF, IP, CoIP
经测试反应性 human, rat
文献引用反应性human, rat, pig, rabbit, canine, zebrafish
免疫原 P53 fusion protein Ag0698 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 tumor protein p53
别名 TP53, Tumour Protein p53, Tumor suppressor p53, tumor protein p53, Antigen NY-CO-13
计算分子量 44 kDa
观测分子量 53 kDa
GenBank蛋白编号BC003596
基因名称 P53
Gene ID (NCBI) 7157
RRIDAB_2881401
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP04637
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

1.       What is p53?

P53 is a tumor suppressor gene that plays a role in maintaining genomic stability and controlling apoptosis. During the cell cycle, it can arrest cells at the G1/S checkpoint and activate DNA repair mechanisms. It is the most mutated gene in cancer. In unstressed cells, p53 usually exists at low levels in an inactive form, being bound to Mdm2.

2.       FAQs and p53

a.       I fail to detect p53 by western blotting

Basal levels of wild-type p53 in untreated cells can be low. Try to load more cell lysate and use a positive control - a lysate of cells treated with DNA-damaging agents should increase p53 levels.

b.      I fail to detect p53 in some cell lines by western blotting

Various p53 mutations are present in cancer cell types. If mutations cause truncations/deletions some monoclonal antibodies may no longer recognize mutated p53. You have more chances of detecting various p53 mutants with our polyclonal antibody.

c.       I can detect more than one band ~50 kDa size / different cell lines give bands at slightly different size

p53 is a subject of post-translational modifications (http://p53.free.fr/p53_info/p53_modifications.html) and more than one isoform may be expressed (http://p53.free.fr/p53_info/p53_isoforms.html). Also, it is possible that your cell line of interest expresses one allele with mutated p53 with altered molecular weight.


实验方案

Product Specific Protocols
WB protocol for P53 antibody 60283-2-IgDownload protocol
IHC protocol for P53 antibody 60283-2-IgDownload protocol
IF protocol for P53 antibody 60283-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Bioact Mater

Chemo-immunotherapy by dual-enzyme responsive peptide self-assembling abolish melanoma

Authors - Yuhan Wang
mouseWB

Nat Commun

Circular RNA encoded MET variant promotes glioblastoma tumorigenesis

Authors - Jian Zhong
mouseWB

Redox Biol

Deficiency of S100 calcium binding protein A9 attenuates vascular dysfunction in aged mice

Authors - Boying Zhao
mouseIHC

Aging (Albany NY)

Whole-transcriptome sequencing analysis reveal mechanisms of Yiqi Huoxue Yangyin (YHY) decoction in ameliorating D-gal-induced cardiac aging

Authors - Xue Wang
WB

J Adv Res

TRPM2-mediated feed-forward loop promotes chondrocyte damage in osteoarthritis via calcium-cGAS-STING-NF-κB pathway

Authors - Kai Sun
humanWB

Clin Transl Med

p53 inhibits OTUD5 transcription to promote GPX4 degradation and induce ferroptosis in gastric cancer

Authors - Junjing Zhang
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