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  • Featured Product
  • KD/KO Validated

P53 Monoclonal antibody, PBS Only (Capture/Detector)

P53 Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, rat

Applications

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

6C4B6

Cat no : 60283-2-PBS

Print datasheet

Synonyms

TP53, Tumour Protein p53, Tumor suppressor p53, tumor protein p53, Antigen NY-CO-13

验证数据展示

  • WB analysis using 60283-2-Ig (same clone as 60283-2-PBS)

    WB analysis using 60283-2-Ig (same clone as 60283-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60283-2-Ig (P53 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60283-2-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 60283-2-Ig (same clone as 60283-2-PBS)

    IHC staining of human colon cancer using 60283-2-Ig (same clone as 60283-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60283-2-Ig (P53 antibody) at dilution of 1:3200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60283-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with P53 antibody included
  • IF Staining of A431 using 60283-2-Ig (same clone as 60283-2-PBS)

    IF Staining of A431 using 60283-2-Ig (same clone as 60283-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using P53 antibody (60283-2-Ig, Clone: 6C4B6 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 60283-2-PBS in a different storage buffer formulation.

  • WB analysis of HSC-T6 using 60283-2-Ig (same clone as 60283-2-PBS)

    WB analysis of HSC-T6 using 60283-2-Ig (same clone as 60283-2-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 60283-2-Ig (P53 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60283-2-PBS in a different storage buffer formulation.

  • WB analysis of ROS1728 using 60283-2-Ig (same clone as 60283-2-PBS)

    WB analysis of ROS1728 using 60283-2-Ig (same clone as 60283-2-PBS)

    ROS1728 cells were subjected to SDS PAGE followed by western blot with 60283-2-Ig (P53 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60283-2-PBS in a different storage buffer formulation.

  • WB analysis of rat thymus using 60283-2-Ig (same clone as 60283-2-PBS)

    WB analysis of rat thymus using 60283-2-Ig (same clone as 60283-2-PBS)

    rat thymus tissue were subjected to SDS PAGE followed by western blot with 60283-2-Ig (P53 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60283-2-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 60283-2-Ig (same clone as 60283-2-PBS)

    IF Staining of A431 using 60283-2-Ig (same clone as 60283-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using P53 antibody (60283-2-Ig, Clone: 6C4B6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60283-2-PBS in a different storage buffer formulation.

  • Cytometric bead array in cell lysate using MP50182-1

    Cytometric bead array in cell lysate using MP50182-1

    Cytometric bead array in cell lysate using MP50182-1, Phospho-P53 (Ser15) Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67826-1-PBS. Detection antibody: 60283-2-PBS. Cell lysate: Non-treated HT-29 and Calyculin A treated HT-29 (30μg/well). Non-related target CEACAM1 Monoclonal Matched Antibody Pair (MP50132-1) was served as control.

  • Cytometric bead array in cell lysate using MP50184-1

    Cytometric bead array in cell lysate using MP50184-1

    Cytometric bead array in cell lysate using MP50184-1, Phospho-P53 (Ser46) Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60283-2-PBS. Detection antibody: 67900-1-PBS. Cell lysate: Non-treated HT-29 and Calyculin A treated HT-29 (30μg/well). Non-related target CEACAM1 Monoclonal Matched Antibody Pair (MP50132-1) was served as control.

Planning an IHC experiment? We recommend our IHCeasy P53 Ready-To-Use IHC Kit. P53 primary antibody included.

产品信息

60283-2-PBS targets P53 as part of a matched antibody pair:

MP50182-1: 67826-1-PBS capture and 60283-2-PBS detection (validated in Cytometric bead array)

MP50184-1: 60283-2-PBS capture and 67900-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human, rat
Immunogen P53 fusion protein Ag0698 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name tumor protein p53
Synonyms TP53, Tumour Protein p53, Tumor suppressor p53, tumor protein p53, Antigen NY-CO-13
Calculated Molecular Weight 44 kDa
Observed Molecular Weight 53 kDa
GenBank Accession NumberBC003596
Gene Symbol P53
Gene ID (NCBI) 7157
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP04637
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

1.       What is p53?

P53 is a tumor suppressor gene that plays a role in maintaining genomic stability and controlling apoptosis. During the cell cycle, it can arrest cells at the G1/S checkpoint and activate DNA repair mechanisms. It is the most mutated gene in cancer. In unstressed cells, p53 usually exists at low levels in an inactive form, being bound to Mdm2.

2.       FAQs and p53

a.       I fail to detect p53 by western blotting

Basal levels of wild-type p53 in untreated cells can be low. Try to load more cell lysate and use a positive control - a lysate of cells treated with DNA-damaging agents should increase p53 levels.

b.      I fail to detect p53 in some cell lines by western blotting

Various p53 mutations are present in cancer cell types. If mutations cause truncations/deletions some monoclonal antibodies may no longer recognize mutated p53. You have more chances of detecting various p53 mutants with our polyclonal antibody.

c.       I can detect more than one band ~50 kDa size / different cell lines give bands at slightly different size

p53 is a subject of post-translational modifications (http://p53.free.fr/p53_info/p53_modifications.html) and more than one isoform may be expressed (http://p53.free.fr/p53_info/p53_isoforms.html). Also, it is possible that your cell line of interest expresses one allele with mutated p53 with altered molecular weight.