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- KD/KO Validated
CoraLite® Plus 488-conjugated PARK2/Parkin Polyclonal antibody
PARK2/Parkin Polyclonal Antibody for IF-P
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF-P
Conjugate
CoraLite® Plus 488 Fluorescent Dye
验证数据展示
经过测试的应用
| Positive IF-P detected in | mouse heart tissue, mouse brain tissue |
Planning an IHC experiment? We recommend our IHCeasy PARK2 Ready-To-Use IHC Kit. PARK2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 14060-1-AP
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-14060 targets PARK2/Parkin in IF-P applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | IF-P Application Description |
| Tested Reactivity | human, mouse, rat |
| Immunogen | PARK2/Parkin fusion protein Ag5092 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | Parkinson disease (autosomal recessive, juvenile) 2, parkin |
| Synonyms | AR JP, LPRS2, PARK2, Parkinson disease protein 2, PDJ, PRKN |
| Calculated Molecular Weight | 52 kDa |
| Observed Molecular Weight | 42-52 kDa |
| GenBank Accession Number | BC022014 |
| Gene Symbol | Parkin |
| Gene ID (NCBI) | 5071 |
| RRID | AB_2919085 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O60260 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Parkin, a RING-type E3 ubiquitin-protein ligase, is involved in the ubiquitination pathway and contributes to protection from neurotoxicity induced by unfolded protein stresses. Its ubiquitin-protein ligase activity promotes the degradation of a variety of proteins including itself. Mutations in Parkin are implicated in the pathogenesis of autosomal recessive familial Parkinson's disease. It has 8 isoforms produced by alternative splicing.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 PARK2/Parkin antibody CL488-14060 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
