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CoraLite®594-conjugated PARK2/Parkin Polyclonal antibody
PARK2/Parkin Polyclonal Antibody for IF/ICC
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF/ICC
Conjugate
CoraLite®594 Fluorescent Dye
验证数据展示
经过测试的应用
Positive IF/ICC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy PARK2 Ready-To-Use IHC Kit. PARK2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 14060-1-AP
推荐稀释比
Application | Dilution |
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Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL594-14060 targets PARK2/Parkin in IF/ICC applications and shows reactivity with human, mouse, rat samples.
Tested Applications | IF/ICC Application Description |
Tested Reactivity | human, mouse, rat |
Immunogen | PARK2/Parkin fusion protein Ag5092 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | Parkinson disease (autosomal recessive, juvenile) 2, parkin |
Synonyms | AR JP, LPRS2, PARK2, Parkinson disease protein 2, PDJ, PRKN |
Calculated Molecular Weight | 52 kDa |
Observed Molecular Weight | 42-52 kDa |
GenBank Accession Number | BC022014 |
Gene Symbol | Parkin |
Gene ID (NCBI) | 5071 |
Conjugate | CoraLite®594 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 588 nm / 604 nm |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | O60260 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Parkin, a RING-type E3 ubiquitin-protein ligase, is involved in the ubiquitination pathway and contributes to protection from neurotoxicity induced by unfolded protein stresses. Its ubiquitin-protein ligase activity promotes the degradation of a variety of proteins including itself. Mutations in Parkin are implicated in the pathogenesis of autosomal recessive familial Parkinson's disease. It has 8 isoforms produced by alternative splicing.
实验方案
Product Specific Protocols | |
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IF protocol for CL594 PARK2/Parkin antibody CL594-14060 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |