CoraLite®594-conjugated PCNA Recombinant antibody

PCNA Uni-rAbTM Recombinant Antibody for IF-P

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF-P

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

240306B9

Cat no : CL594-81302-6

Print datasheet

Synonyms



经过测试的应用

Positive IF-P detected inmouse testis tissue
Planning an IHC experiment? We recommend our IHCeasy PCNA Ready-To-Use IHC Kit. PCNA primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 81302-6-PBS

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of CL594-81302-6 is PCNA Fusion Protein expressed in E. coli.

Tested Applications IF-P Application Description
Tested Reactivity human, mouse, rat
Immunogen PCNA fusion protein Ag0277 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name proliferating cell nuclear antigen
Synonyms
Calculated Molecular Weight 29 kDa/31 kDa
Observed Molecular Weight 36 kDa
GenBank Accession NumberBC000491
Gene Symbol PCNA
Gene ID (NCBI) 5111
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP12004
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Proliferating Cell Nuclear Antigen, commonly known as PCNA, is a protein that acts as a processivity factor for DNA polymerase δ in eukaryotic cells. This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. PCNA induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. It has to be loaded onto DNA in order to be able to stimulate APEX2. PCNA protein is highly conserved during evolution; the deduced amino acid sequences of rat and human differ by only 4 of 261 amino acids. PCNA has been used as loading control for proliferating cells. The calculated molecular weight of PCNA is 29 kDa, but modified PCNA is 36kDa (PMID: 1358458).

实验方案

Product Specific Protocols
IF protocol for CL594 PCNA antibody CL594-81302-6Download protocol
Standard Protocols
Click here to view our Standard Protocols