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CoraLite® Plus 488-conjugated PERK/EIF2AK3 Polyclonal antibody
PERK/EIF2AK3 Polyclonal Antibody for FC (Intra)
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
验证数据展示
经过测试的应用
Positive FC (Intra) detected in | HepG2 cells |
Positive FC detected in | HepG2 cells |
For other applications, we recommend the unconjugated version of this antibody, 20582-1-AP
推荐稀释比
Application | Dilution |
---|---|
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
Flow Cytometry (FC) | FC : 0.80 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL488-20582 targets PERK/EIF2AK3 in FC (Intra) applications and shows reactivity with human samples.
Tested Applications | FC (Intra) Application Description |
Tested Reactivity | human |
Immunogen | Peptide 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | eukaryotic translation initiation factor 2-alpha kinase 3 |
Synonyms | DKFZp781H1925, EIF2AK3, HRI, HsPEK, Pancreatic eIF2 alpha kinase, PEK, PERK, PERK/EIF2AK3, WRS |
Calculated Molecular Weight | 125 kDa |
Observed Molecular Weight | 140 kDa |
GenBank Accession Number | NM_004836 |
Gene Symbol | PERK |
Gene ID (NCBI) | 9451 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q9NZJ5 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PERK, also named as EIF2AK3 or PEK, belongs to the protein kinase superfamily, Ser/Thr protein kinase family and GCN2 subfamily. It acts potentially as a metabolic sensor in the INS-secreting beta-cells to modulate the trafficking and quality control of pro-INS in the ER relative to the physiological demands for circulating INS (PMID:20530744). PERK and EIF2AK4 also have a functional role on regulating translation under non-stressed conditions, apart from their long established roles as stress kinases (PMID: 21638082). Defects in EIF2AK3 are the cause of Wolcott-Rallison syndrome (WRS). The molecular mass of PERK is 125 kDa and can be detected as 140 kDa or 170 kDa due to some modifications such as glycosylation and phosphorylation (PMID: 20851775, 24371088, 21703327). This antibody is specific to PERK.
实验方案
Product Specific Protocols | |
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FC protocol for CL Plus 488 PERK/EIF2AK3 antibody CL488-20582 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |