验证数据展示
经过测试的应用
| Positive FC (Intra) detected in | A431 cells |
Planning an IHC experiment? We recommend our IHCeasy RAB7A Ready-To-Use IHC Kit. RAB7A primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 55469-1-AP
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-55469 targets RAB7A in FC (Intra) applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | FC (Intra) Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | RAB7A, member RAS oncogene family |
| 别名 | RAB7, Ras-related protein Rab-7a, Ras related protein Rab 7a, EC:3.6.5.2 |
| 计算分子量 | 23 kDa |
| 观测分子量 | 23 kDa |
| GenBank蛋白编号 | NM_004637 |
| 基因名称 | RAB7A |
| Gene ID (NCBI) | 7879 |
| RRID | AB_3084130 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P51149 |
| 储存缓冲液 | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RAB7A, also named as RAB7, belongs to the small GTPase superfamily and Rab family. It is involved in late endocytic transport. RAB7A contributes to the maturation of phagosomes (acidification). Defects in RAB7A are the cause of Charcot-Marie-Tooth disease type 2B (CMT2B). This antibody is specific to RAB7A.
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 488 RAB7A antibody CL488-55469 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |