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KD/KO Validated

RBM15 Polyclonal antibody

RBM15 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA and More (3)

Conjugate

Unconjugated

Publications

Cat no : 10587-1-AP

Print datasheet

Synonyms

RNA-binding protein 15, RNA-binding motif protein 15, RBM 15, OTT1, OTT

验证数据展示

IP experiment of HEK-293 using 10587-1-AP
IP result of anti-RBM15 (IP:10587-1-AP, 4ug; Detection:10587-1-AP 1:500) with HEK-293 cells lysate 2600 ug.
IHC staining of human breast cancer using 10587-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10587-1-AP (RBM15 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with RBM15 antibody included
WB analysis of HEK293 cells using 10587-1-AP
WB result of RBM15 antibody (10587-1-AP, 1:3000) with si-control and si-RBM15 transfected HEK293 cells.
WB analysis of HEK-293 using 10587-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 10587-1-AP (RBM15 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of SW480 using 10587-1-AP
SW480 cells were subjected to SDS PAGE followed by western blot with 10587-1-AP (RBM15 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis of SW480 using 10587-1-AP
SW480 cells were subjected to SDS PAGE followed by western blot with 10587-1-AP (RBM15 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
IF Staining of A431 using 10587-1-AP
Immunofluorescent analysis of (4% PFA) fixed A431 cells using RBM15 antibody (10587-1-AP) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
IF Staining of HepG2 using 10587-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RBM15 antibody (10587-1-AP) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

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Cat No. 10587-1-AP

Size

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经过测试的应用

Positive WB detected in	SW480 cells, HEK293 cells, HEK-293 cells
Positive IP detected in	HEK-293 cells
Positive IHC detected in	human breast cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	A431 cells, HepG2 cells

Planning an IHC experiment? We recommend our IHCeasy RBM15 Ready-To-Use IHC Kit. RBM15 primary antibody included.

Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:16000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 11 publications below
WB	See 38 publications below
IHC	See 11 publications below
IF	See 6 publications below
IP	See 4 publications below
CoIP	See 2 publications below
RIP	See 1 publications below

产品信息

10587-1-AP targets RBM15 in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications	WB, IHC, IF/ICC, IP, ELISA Application Description
Cited Applications	WB, IHC, IF, IP, CoIP, RIP
Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Immunogen	RBM15 fusion protein Ag0938 种属同源性预测
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Full Name	RNA binding motif protein 15
Synonyms	RNA-binding protein 15, RNA-binding motif protein 15, RBM 15, OTT1, OTT
Calculated Molecular Weight	105 kDa
Observed Molecular Weight	100-107 kDa
GenBank Accession Number	BC006397
Gene Symbol	RBM15
Gene ID (NCBI)	64783
RRID	AB_2175757
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q96T37
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

RNA-binding motif protein 15 (RBM15) belongs to the SPEN protein family, which have repressor function in several signaling pathway, and may bind to RNA through interaction with spliceosome components. It was first described as a 5′ translocation partner of the MAL gene in t(1,22)(p13;q13) infant acute megakaryocytic leukemia, and it functions in murine hematopoiesis through modulating Notch-induced transcriptional activation, which play crucial roles in leukemogenesis. Decreasing RBM15 levels with RNA interference could inhibit the growth and proliferation, block the cell cycle, and induce apoptosis in CML cells.

实验方案

Product Specific Protocols
WB protocol for RBM15 antibody 10587-1-AP	Download protocol
IHC protocol for RBM15 antibody 10587-1-AP	Download protocol
IF protocol for RBM15 antibody 10587-1-AP	Download protocol
IP protocol for RBM15 antibody 10587-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
mouse	WB	Nature METTL3 regulates heterochromatin in mouse embryonic stem cells. Authors - Wenqi Xu KO Validated View Article
human	WB,IF	Mol Cancer Co-transcriptional R-loops-mediated epigenetic regulation drives growth retardation and docetaxel chemosensitivity enhancement in advanced prostate cancer Authors - Yufan Ying View Article
human	WB	Mol Cell mTORC1 stimulates cell growth through SAM synthesis and m6A mRNA-dependent control of protein synthesis. Authors - Elodie Villa View Article
human	WB,IF,RIP,CoIP	Dev Cell N6-methyladenosine modification of MALAT1 promotes metastasis via reshaping nuclear speckles. Authors - Xinyu Wang KD Validated View Article
human	WB	Elife Cross-talk between PRMT1-mediated methylation and ubiquitylation on RBM15 controls RNA splicing. Authors - Li Zhang View Article
human	WB,IF,IP	Nucleic Acids Res Nuclear export factor RBM15 facilitates the access of DBP5 to mRNA. Authors - Zolotukhin Andrei S AS View Article

RBM15 Polyclonal antibody

RBM15 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

验证数据展示

IP experiment of HEK-293 using 10587-1-AP

IHC staining of human breast cancer using 10587-1-AP

WB analysis of HEK293 cells using 10587-1-AP

WB analysis of HEK-293 using 10587-1-AP

WB analysis of SW480 using 10587-1-AP

WB analysis of SW480 using 10587-1-AP

IF Staining of A431 using 10587-1-AP

IF Staining of HepG2 using 10587-1-AP

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

METTL3 regulates heterochromatin in mouse embryonic stem cells.

Co-transcriptional R-loops-mediated epigenetic regulation drives growth retardation and docetaxel chemosensitivity enhancement in advanced prostate cancer

mTORC1 stimulates cell growth through SAM synthesis and m6A mRNA-dependent control of protein synthesis.

N6-methyladenosine modification of MALAT1 promotes metastasis via reshaping nuclear speckles.

Cross-talk between PRMT1-mediated methylation and ubiquitylation on RBM15 controls RNA splicing.

Nuclear export factor RBM15 facilitates the access of DBP5 to mRNA.