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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated RBM15 Polyclonal antibody

RBM15 Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

Cat no : CL594-10587

Print datasheet

Synonyms

FLJ12479, FLJ21943, One twenty two protein 1, OTT, OTT1, RBM15, RNA binding motif protein 15, SPEN

验证数据展示

  • IF Staining of HepG2 using CL594-10587

    IF Staining of HepG2 using CL594-10587

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®594 RBM15 antibody (CL594-10587) at dilution of 1:200.

经过测试的应用

Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy RBM15 Ready-To-Use IHC Kit. RBM15 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 10587-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-10587 targets RBM15 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human, mouse, rat
Immunogen RBM15 fusion protein Ag0938 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name RNA binding motif protein 15
Synonyms FLJ12479, FLJ21943, One twenty two protein 1, OTT, OTT1, RBM15, RNA binding motif protein 15, SPEN
Calculated Molecular Weight 105 kDa
Observed Molecular Weight 100-107 kDa
GenBank Accession NumberBC006397
Gene Symbol RBM15
Gene ID (NCBI) 64783
RRIDAB_2919738
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96T37
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RNA-binding motif protein 15 (RBM15) belongs to the SPEN protein family, which have repressor function in several signaling pathway, and may bind to RNA through interaction with spliceosome components. It was first described as a 5′ translocation partner of the MAL gene in t(1,22)(p13;q13) infant acute megakaryocytic leukemia, and it functions in murine hematopoiesis through modulating Notch-induced transcriptional activation, which play crucial roles in leukemogenesis. Decreasing RBM15 levels with RNA interference could inhibit the growth and proliferation, block the cell cycle, and induce apoptosis in CML cells.

实验方案

Product Specific Protocols
IF protocol for CL594 RBM15 antibody CL594-10587Download protocol
Standard Protocols
Click here to view our Standard Protocols