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CoraLite® Plus 488-conjugated RPA2 Polyclonal antibody

RPA2 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-10412

Print datasheet

Synonyms

RP A p34, RP A p32, RF-A protein 2, replication protein A2, 32kDa, Replication factor A protein 2

验证数据展示

  • IF Staining of A431 using CL488-10412

    IF Staining of A431 using CL488-10412

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CoraLite® Plus 488 RPA2 antibody (CL488-10412) at dilution of 1:200, CL594-Phalloidin (red).

  • FC experiment of U2OS using CL488-10412

    FC experiment of U2OS using CL488-10412

    1x10^6 U2OS cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Rpa2 Polyclonal Antibody (CL488-10412)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF/ICC detected inA431 cells
Positive FC (Intra) detected inU2OS cells
For other applications, we recommend the unconjugated version of this antibody, 10412-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-10412 targets RPA2 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human
Immunogen RPA2 fusion protein Ag0690 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name replication protein A2, 32kDa
Synonyms RP A p34, RP A p32, RF-A protein 2, replication protein A2, 32kDa, Replication factor A protein 2
Calculated Molecular Weight 29 kDa
Observed Molecular Weight 32 kDa
GenBank Accession NumberBC001630
Gene Symbol RPA2
Gene ID (NCBI) 6118
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP15927
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RPA2, a subunit of the heterotrimeric replication protein A (RPA) complex, is overexpressed in various cancers. RPA2 is a major target for upstream kinases, including CDKs and the three PIKKs (ATR, ataxia-telangiectasia mutated [ATM], and DNA-PK). RPA2 comprises multiple critical Ser/Tr residues that are phosphorylated in sequential order in response to genotoxic stress.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 RPA2 antibody CL488-10412Download protocol
Standard Protocols
Click here to view our Standard Protocols