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验证数据展示

  • IF Staining of RAW 264.7 using CL594-66489

    IF Staining of RAW 264.7 using CL594-66489

    Immunofluorescent analysis of (-20°C Ethanol) fixed RAW 264.7 cells using CoraLite®594 S100A4 antibody (CL594-66489, Clone: 2G11B4 ) at dilution of 1:200.

  • FC experiment of HeLa using CL594-66489

    FC experiment of HeLa using CL594-66489

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite®594-conjugated S100A4 Monoclonal antibody (CL594-66489, Clone:2G11B4)(red), or 0.8 ug CoraLite®594 Mouse IgG2a isotype control (11A1B2) (CL594-66360-2, Clone: 11A1B2) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated S100A4 Monoclonal antibody

S100A4 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL594-66489
CloneNo. 2G11B4

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

IF/ICC, FC (Intra)

Synonyms

Fibroblast-specific protein-1, Fibroblast-specific protein 1, Fibroblast Specific Protein, CAPL, Calvasculin

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inRAW 264.7 cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy S100A4 Ready-To-Use IHC Kit. S100A4 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66489-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66489 targets S100A4 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse
Immunogen S100A4 fusion protein Ag9019 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name S100 calcium binding protein A4
Synonyms Fibroblast-specific protein-1, Fibroblast-specific protein 1, Fibroblast Specific Protein, CAPL, Calvasculin
Calculated Molecular Weight 101 aa, 12 kDa
GenBank Accession NumberBC016300
Gene Symbol S100A4
Gene ID (NCBI) 6275
ENSEMBL Gene IDENSG00000196154
RRIDAB_2883593
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP26447
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

S100A4 is a member of the S100 family of calcium-binding proteins. The S100 family members have been involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100A4 is known to localize to and function in the nucleus, cytoplasm of cells, and the extracellular space. S100A4 has also been shown to be associated with tumor growth, motility, invasion, metastasis, angiogenesis, apoptosis, and chemoresistance. It is a fibroblast-specific protein associated with mesenchymal cell morphology and motility, is expressed during epithelial-mesenchymal transformations (EMT) in vivo (PMID: 9362334). It is a specific prognostic marker for renal survival in patients with IgAN (PMID: 16105038). It is also an improved marker for lung fibroblasts that could be useful for investigating the pathogenesis of pulmonary fibrosis(PMID: 15618458). Overexpression of S100A4 is correlated with a worse prognosis inpatients with various types of cancer.

实验方案

Product Specific Protocols
IF protocol for CL594 S100A4 antibody CL594-66489Download protocol
FC protocol for CL594 S100A4 antibody CL594-66489Download protocol
Standard Protocols
Click here to view our Standard Protocols