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CoraLite® Plus 647-conjugated S100A4 Monoclonal antibody
S100A4 Monoclonal Antibody for IF/ICC
Host / Isotype
Mouse / IgG2a
Reactivity
Human, mouse
Applications
IF/ICC
Conjugate
CoraLite® Plus 647 Fluorescent Dye
CloneNo.
2G11B4
验证数据展示
经过测试的应用
Positive IF/ICC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy S100A4 Ready-To-Use IHC Kit. S100A4 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66489-1-Ig
推荐稀释比
Application | Dilution |
---|---|
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL647-66489 targets S100A4 in IF/ICC applications and shows reactivity with Human, mouse samples.
Tested Applications | IF/ICC Application Description |
Tested Reactivity | Human, mouse |
Immunogen | S100A4 fusion protein Ag9019 种属同源性预测 |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Full Name | S100 calcium binding protein A4 |
Synonyms | 18A2, 42A, Calvasculin, CAPL, Fibroblast-specific protein 1, FSP1, FSP-1, Metastasin, MTS1, P9KA, PEL98, Protein Mts1, Protein S100 A4, S100A4 |
Calculated Molecular Weight | 101 aa, 12 kDa |
Observed Molecular Weight | 12 kDa |
GenBank Accession Number | BC016300 |
Gene Symbol | S100A4 |
Gene ID (NCBI) | 6275 |
ENSEMBL Gene ID | ENSG00000196154 |
Conjugate | CoraLite® Plus 647 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 654 nm / 674 nm |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P26447 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage. |
背景介绍
S100A4 is a member of the S100 family of calcium-binding proteins. The S100 family members have been involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100A4 is known to localize to and function in the nucleus, cytoplasm of cells, and the extracellular space. S100A4 has also been shown to be associated with tumor growth, motility, invasion, metastasis, angiogenesis, apoptosis, and chemoresistance. It is a fibroblast-specific protein associated with mesenchymal cell morphology and motility, is expressed during epithelial-mesenchymal transformations (EMT) in vivo (PMID: 9362334). It is a specific prognostic marker for renal survival in patients with IgAN (PMID: 16105038). It is also an improved marker for lung fibroblasts that could be useful for investigating the pathogenesis of pulmonary fibrosis(PMID: 15618458). Overexpression of S100A4 is correlated with a worse prognosis inpatients with various types of cancer.
实验方案
Product Specific Protocols | |
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IF protocol for CL Plus 647 S100A4 antibody CL647-66489 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |