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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated SOX2 Monoclonal antibody

SOX2 Monoclonal Antibody for IF-P

Host / Isotype

Mouse / IgG1

Reactivity

human, pig, mouse

Applications

IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

4B9B10

Cat no : CL488-66411

Print datasheet

Synonyms

ANOP3, MCOPS3, SOX2, Transcription factor SOX 2

验证数据展示

  • IF Staining of mouse brain using CL488-66411

    IF Staining of mouse brain using CL488-66411

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CL488-66411 (SOX2 antibody) at dilution of 1:50.

经过测试的应用

Positive IF-P detected inmouse brain tissue
Planning an IHC experiment? We recommend our IHCeasy SOX2 Ready-To-Use IHC Kit. SOX2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66411-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66411 targets SOX2 in IF-P applications and shows reactivity with human, pig, mouse samples.

Tested Applications IF-P Application Description
Tested Reactivity human, pig, mouse
Immunogen SOX2 fusion protein Ag13635 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name SRY (sex determining region Y)-box 2
Synonyms ANOP3, MCOPS3, SOX2, Transcription factor SOX 2
Calculated Molecular Weight 34 kDa
Observed Molecular Weight 34-40 kDa
GenBank Accession NumberBC013923
Gene Symbol SOX2
Gene ID (NCBI) 6657
RRIDAB_2883330
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP48431
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Sox2, also known as SRY (sex determining region Y)-box 2, is a transcription factor essential for maintaining self-renewal of undifferentiated ES cells and is one of the key transcription factors used to reprogram mouse and human fibroblasts to a pluripotent state. Sox2 expressed in undifferentiated pluripotent stem cells and germ cells during development.. This antibody is raised against an internal region of human SOX2. And the antibody is conjugated with CL488, Ex/Em 488 nm/515 nm.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 SOX2 antibody CL488-66411Download protocol
Standard Protocols
Click here to view our Standard Protocols