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验证数据展示

  • IF Staining of mouse brain using CL594-66411

    IF Staining of mouse brain using CL594-66411

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CL594-66411 (SOX2 antibody) at dilution of 1:50.

  • IF Staining of rat brain using CL594-66411

    IF Staining of rat brain using CL594-66411

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using CoraLite®594 SOX2 antibody (CL594-66411, Clone: 4B9B10 ) at dilution of 1:400.

  • IF Staining of U-251 using CL594-66411

    IF Staining of U-251 using CL594-66411

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using CoraLite®594 SOX2 antibody (CL594-66411, Clone: 4B9B10 ) at dilution of 1:200.

  • FC experiment of U-251 using CL594-66411

    FC experiment of U-251 using CL594-66411

    1X10^6 U-251 cells were intracellularly stained with 0.2 ug CoraLite®594 Anti-Human SOX2 (CL594-66411, Clone:4B9B10) (red), or 0.2 ug Mouse IgG1 Isotype Control (CL594-66360, Clone: T1F8D3F10) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • IF Staining of mouse brain using CL594-66411

    IF Staining of mouse brain using CL594-66411

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite®594 SOX2 antibody (CL594-66411, Clone: 4B9B10 ) at dilution of 1:200. DAPI (blue).

  • Featured Product
  • KD/KO验证

CoraLite®594-conjugated SOX2 Monoclonal antibody

SOX2 Monoclonal Antibody for IF/ICC, IF-P, FC (Intra)
Cat No. CL594-66411

产品说明书

CloneNo. 4B9B10

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

IF/ICC, IF-P, FC (Intra)

别名

Transcription factor SOX 2, SOX 2, MCOPS3, MCOPS 3, ANOP3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive IF-P detected inmouse brain tissue, rat brain tissue
Positive IF/ICC detected inU-251 cells
Positive FC (Intra) detected inU-251 cells
Planning an IHC experiment? We recommend our IHCeasy SOX2 Ready-To-Use IHC Kit. SOX2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66411-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66411 targets SOX2 in IF/ICC, IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, IF-P, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 SOX2 fusion protein Ag13635 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 SRY (sex determining region Y)-box 2
别名 Transcription factor SOX 2, SOX 2, MCOPS3, MCOPS 3, ANOP3
计算分子量 34 kDa
观测分子量 34-40 kDa
GenBank蛋白编号BC013923
基因名称 SOX2
Gene ID (NCBI) 6657
RRIDAB_2883578
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP48431
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Sox2, also known as SRY (sex determining region Y)-box 2, is a transcription factor essential for maintaining self-renewal of undifferentiated ES cells and is one of the key transcription factors used to reprogram mouse and human fibroblasts to a pluripotent state. Sox2 expressed in undifferentiated pluripotent stem cells and germ cells during development. This antibody is raised against an internal region of human SOX2. And the antibody is conjugated with CL594, Ex/Em 593 nm/614 nm.

实验方案

Product Specific Protocols
IF protocol for CL594 SOX2 antibody CL594-66411Download protocol
Standard Protocols
Click here to view our Standard Protocols