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CoraLite® Plus 488-conjugated TGFBI / BIGH3 Monoclonal antibody
TGFBI / BIGH3 Monoclonal Antibody for IF-P, FC (Intra)
Host / Isotype
Mouse / IgG2a
Reactivity
human
Applications
IF-P, FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
3E11D11
验证数据展示
经过测试的应用
| Positive IF-P detected in | human colon cancer tissue |
| Positive FC (Intra) detected in | Y79 cells |
| Positive FC detected in | Y79 cells |
Planning an IHC experiment? We recommend our IHCeasy TGFBI Ready-To-Use IHC Kit. TGFBI primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 60007-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-60007 targets TGFBI / BIGH3 in IF-P, FC (Intra) applications and shows reactivity with human samples.
| Tested Applications | IF-P, FC (Intra) Application Description |
| Tested Reactivity | human |
| Immunogen | TGFBI / BIGH3 fusion protein Ag0241 种属同源性预测 |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | transforming growth factor, beta-induced, 68kDa |
| Synonyms | Beta ig h3, BIGH3, CDB1, CDG2, CDGG1, CSD, CSD1, CSD2, CSD3, EBMD, Kerato epithelin, LCD1, RGD CAP, TGFBI, TGFBI / BIGH3 |
| Calculated Molecular Weight | 683 aa, 75 kDa |
| GenBank Accession Number | BC000097 |
| Gene Symbol | TGFBI |
| Gene ID (NCBI) | 7045 |
| ENSEMBL Gene ID | ENSG00000120708 |
| RRID | AB_2919225 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q15582 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TGFBI, also named as BIGH3, Kerato-epithelin and RGD-CAP, binds to type I, II, and IV collagens. TGFBI is an adhesion protein which may play an important role in cell-collagen interactions. In cartilage, it may be involved in endochondral bone formation. TGFBI is an extracellular matrix adaptor protein, it has been reported to be differentially expressed in transformed tissues. TGFBI is a predictive factor of the response to chemotherapy, and suggest the use of TGFBI-derived peptides as possible therapeutic adjuvants for the enhancement of responses to chemotherapy.(PMID:20509890) Defects in TGFBI are the cause of epithelial basement membrane corneal dystrophy (EBMD). Defects in TGFBI are the cause of corneal dystrophy Groenouw type 1 (CDGG1). Defects in TGFBI are the cause of corneal dystrophy lattice type 1 (CDL1). Defects in TGFBI are a cause of corneal dystrophy Thiel-Behnke type (CDTB). Defects in TGFBI are the cause of Reis-Buecklers corneal dystrophy (CDRB). Defects in TGFBI are the cause of lattice corneal dystrophy type 3A (CDL3A). Defects in TGFBI are the cause of Avellino corneal dystrophy (ACD).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 TGFBI / BIGH3 antibody CL488-60007 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
