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验证数据展示

  • IF Staining of human colon cancer using CL594-60007

    IF Staining of human colon cancer using CL594-60007

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CoraLite®594 TGFBI / BIGH3 antibody (CL594-60007, Clone: 3E11D11 ) at dilution of 1:200.

  • IF Staining of human colon cancer using CL594-60007

    IF Staining of human colon cancer using CL594-60007

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CoraLite®594 TGFBI / BIGH3 antibody (CL594-60007, Clone: 3E11D11 ) at dilution of 1:200.

  • FC experiment of Y79 using CL594-60007

    FC experiment of Y79 using CL594-60007

    1X10^6 Y79 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human TGFBI / BIGH3 (CL594-60007, Clone:3E11D11) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

CoraLite®594-conjugated TGFBI / BIGH3 Monoclonal antibody

TGFBI / BIGH3 Monoclonal Antibody for IF-P, FC (Intra)
Cat No. CL594-60007

产品说明书

CloneNo. 3E11D11

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

IF-P, FC (Intra)

别名

Beta ig h3, BIGH3, CDB1, CDG2, CDGG1, CSD, CSD1, CSD2, CSD3, EBMD, Kerato epithelin, LCD1, RGD CAP, TGFBI, TGFBI / BIGH3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive IF-P detected inhuman colon cancer tissue
Positive FC (Intra) detected inY79 cells
Positive FC detected inY79 cells
Planning an IHC experiment? We recommend our IHCeasy TGFBI Ready-To-Use IHC Kit. TGFBI primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 60007-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-60007 targets TGFBI / BIGH3 in IF-P, FC (Intra) applications and shows reactivity with human samples.

经测试应用 IF-P, FC (Intra) Application Description
经测试反应性 human
免疫原 TGFBI / BIGH3 fusion protein Ag0241 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 transforming growth factor, beta-induced, 68kDa
别名 Beta ig h3, BIGH3, CDB1, CDG2, CDGG1, CSD, CSD1, CSD2, CSD3, EBMD, Kerato epithelin, LCD1, RGD CAP, TGFBI, TGFBI / BIGH3
计算分子量 683 aa, 75 kDa
GenBank蛋白编号BC000097
基因名称 TGFBI
Gene ID (NCBI) 7045
ENSEMBL Gene IDENSG00000120708
RRIDAB_2919887
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ15582
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TGFBI, also named as BIGH3, Kerato-epithelin and RGD-CAP, binds to type I, II, and IV collagens. TGFBI is an adhesion protein which may play an important role in cell-collagen interactions. In cartilage, it may be involved in endochondral bone formation. TGFBI is an extracellular matrix adaptor protein, it has been reported to be differentially expressed in transformed tissues. TGFBI is a predictive factor of the response to chemotherapy, and suggest the use of TGFBI-derived peptides as possible therapeutic adjuvants for the enhancement of responses to chemotherapy.(PMID:20509890) Defects in TGFBI are the cause of epithelial basement membrane corneal dystrophy (EBMD). Defects in TGFBI are the cause of corneal dystrophy Groenouw type 1 (CDGG1). Defects in TGFBI are the cause of corneal dystrophy lattice type 1 (CDL1). Defects in TGFBI are a cause of corneal dystrophy Thiel-Behnke type (CDTB). Defects in TGFBI are the cause of Reis-Buecklers corneal dystrophy (CDRB). Defects in TGFBI are the cause of lattice corneal dystrophy type 3A (CDL3A). Defects in TGFBI are the cause of Avellino corneal dystrophy (ACD).

实验方案

Product Specific Protocols
IF protocol for CL594 TGFBI / BIGH3 antibody CL594-60007Download protocol
Standard Protocols
Click here to view our Standard Protocols