• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • IF Staining of HUVEC using CL488-10694

    IF Staining of HUVEC using CL488-10694

    Immunofluorescent analysis of (-20°C Ethanol) fixed oleic acid treated HUVEC cells using CoraLite® Plus 488 TIP47 antibody (CL488-10694) at dilution of 1:200.

  • IF Staining of HeLa using CL488-10694

    IF Staining of HeLa using CL488-10694

    Immunofluorescent analysis of (-20°C Ethanol) fixed oleic acid treated HeLa cells using CoraLite® Plus 488 TIP47 antibody (CL488-10694) at dilution of 1:200, CL594-Phalloidin (red).

  • FC experiment of HeLa using CL488-10694

    FC experiment of HeLa using CL488-10694

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human TIP47 (CL488-10694) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated Perilipin 3/TIP47 Polyclonal antibody

Perilipin 3/TIP47 Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-10694

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra)

别名

M6PRBP1, Perilipin 3, TIP47, Perilipin-3, Mannose-6-phosphate receptor-binding protein 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488

规格: 
100 μL

¥3200/ 100 μL

浓度: 1000 ug/ml

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive IF/ICC detected inoleic acid treated HeLa cells, oleic acid treated HUVEC cells
Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 10694-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-10694 targets Perilipin 3/TIP47 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human
免疫原 Perilipin 3/TIP47 fusion protein Ag1028 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 mannose-6-phosphate receptor binding protein 1
别名 M6PRBP1, Perilipin 3, TIP47, Perilipin-3, Mannose-6-phosphate receptor-binding protein 1
计算分子量 47 kDa
观测分子量 47 kDa
GenBank蛋白编号BC007566
基因名称 TIP47/PLIN3
Gene ID (NCBI) 10226
RRIDAB_2918992
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO60664
储存缓冲液 PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Mannose 6-phosphate receptors (M6PRs) transport newly synthesized lysosomal hydrolases from the Golgi to prelysosomes and then return to the Golgi for another round of transport. M6PRBP1 (mannose-6-phosphate receptor binding protein 1), also known as TIP47, PLIN3 or PP17, interacts with the cytoplasmic domains of both cation-independent and cation-dependent M6PRs, and is required for endosome-to-Golgi transport. In addition to M6PR recycling, M6PRBP1 plays a role in lipid droplet biogenesis, and is also implicated in rhodopsin photobleaching and viral infection. M6PRBP1 has been found to be expressed in a variety of human tissues (including colon, liver and lung parenchyme, mammary gland, and skin) and is overexpressed in certain cancer cell lines. It binds to lipid droplets and also occurs in cytosol and on endosomal membranes.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Perilipin 3/TIP47 antibody CL488-10694Download protocol
Standard Protocols
Click here to view our Standard Protocols