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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 647-conjugated TIP47 Polyclonal antibody

TIP47 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

Cat no : CL647-10694

Print datasheet

Synonyms

M6PRBP1, Perilipin 3, Perilipin-3, Mannose-6-phosphate receptor-binding protein 1, 47 kDa MPR-binding protein

验证数据展示

  • IF Staining of HeLa using CL647-10694

    IF Staining of HeLa using CL647-10694

    Immunofluorescent analysis of (-20°C Ethanol) fixed oleic acid treated HeLa cells using CoraLite® Plus 647 TIP47 antibody (CL647-10694) at dilution of 1:200.

  • FC experiment of HeLa using CL647-10694

    FC experiment of HeLa using CL647-10694

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 647-conjugated TIP47 Polyclonal antibody (CL647-10694)(red), or 0.4 ug CoraLite® Plus 647-conjugated Rabbit IgG control Rabbit PolyAb (CL647-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF/ICC detected inoleic acid treated HeLa cells
Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 10694-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-10694 targets TIP47 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human
Immunogen TIP47 fusion protein Ag1028 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name mannose-6-phosphate receptor binding protein 1
Synonyms M6PRBP1, Perilipin 3, Perilipin-3, Mannose-6-phosphate receptor-binding protein 1, 47 kDa MPR-binding protein
Calculated Molecular Weight 47 kDa
Observed Molecular Weight 47 kDa
GenBank Accession NumberBC007566
Gene Symbol TIP47/PLIN3
Gene ID (NCBI) 10226
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO60664
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Mannose 6-phosphate receptors (M6PRs) transport newly synthesized lysosomal hydrolases from the Golgi to prelysosomes and then return to the Golgi for another round of transport. M6PRBP1 (mannose-6-phosphate receptor binding protein 1), also known as TIP47, PLIN3 or PP17, interacts with the cytoplasmic domains of both cation-independent and cation-dependent M6PRs, and is required for endosome-to-Golgi transport. In addition to M6PR recycling, M6PRBP1 plays a role in lipid droplet biogenesis, and is also implicated in rhodopsin photobleaching and viral infection. M6PRBP1 has been found to be expressed in a variety of human tissues (including colon, liver and lung parenchyme, mammary gland, and skin) and is overexpressed in certain cancer cell lines. It binds to lipid droplets and also occurs in cytosol and on endosomal membranes.

实验方案

Product Specific Protocols
IF protocol for CL Plus 647 TIP47 antibody CL647-10694Download protocol
Standard Protocols
Click here to view our Standard Protocols