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CoraLite®555-conjugated TNP1 Polyclonal antibody

TNP1 Polyclonal Antibody for IF-P, IF-Fro

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF-P, IF-Fro

Conjugate

CoraLite®555 Fluorescent Dye

Cat no : CL555-17178

Print datasheet

Synonyms

STP 1, TNP1, TP 1, TP1

验证数据展示

  • IF Staining of mouse testis using CL555-17178

    IF Staining of mouse testis using CL555-17178

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CoraLite®555 TNP1 antibody (CL555-17178) at dilution of 1:100.

  • IF Staining of mouse testis using CL555-17178

    IF Staining of mouse testis using CL555-17178

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CoraLite®555 TNP1 antibody (CL555-17178) at dilution of 1:100, CoraLite® Plus 488 BOULE antibody (CL488-13720, green), STAR antibody (67130-1-Ig, Clone: 1G6E6, Magenta).

经过测试的应用

Positive IF-P detected inmouse testis tissue
Positive IF-Fro detected inmouse testis tissue
For other applications, we recommend the unconjugated version of this antibody, 17178-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)-FROIF-FRO : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL555-17178 targets TNP1 in IF-P, IF-Fro applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF-P, IF-Fro Application Description
Tested Reactivity human, mouse, rat
Immunogen TNP1 fusion protein Ag10890 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name transition protein 1 (during histone to protamine replacement)
Synonyms STP 1, TNP1, TP 1, TP1
Calculated Molecular Weight 55 aa, 7 kDa
Observed Molecular Weight 6-10 kDa
GenBank Accession NumberBC029516
Gene Symbol TNP1
Gene ID (NCBI) 7141
RRIDAB_2919633
Conjugate CoraLite®555 Fluorescent Dye
Excitation/Emission Maxima Wavelengths557 nm / 570 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP09430
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Transition nuclear proteins (TNP1 and TNP2) are the major nuclear proteins that replace somatic histones during spermatogenesis. TNPs are required for normal chromatin condensation and functional sperm development, spermatogenesis was found to be compromised in both Tnp1 and Tnp2 null mice. TNP1, or TP1, localized in nucleus, is a spermatid-specific product of the haploid genome which replaces histone and is itself replaced in the mature sperm by the protamines. Recently, TNP-1 was used as a germ cell marker in condensing spermatids.

实验方案

Product Specific Protocols
IF protocol for CL555 TNP1 antibody CL555-17178Download protocol
Standard Protocols
Click here to view our Standard Protocols