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CoraLite® Plus 750-conjugated TOM20 Monoclonal antibody

TOM20 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

FC (Intra)

Conjugate

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

1D6F5

Cat no : CL750-66777

Print datasheet

Synonyms

TOMM20, TOMM 20, tom 20, MOM19, MAS20

验证数据展示

  • FC experiment of HeLa using CL750-66777

    FC experiment of HeLa using CL750-66777

    1X10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 750 Anti-Human TOM20 (CL750-66777, Clone:1D6F5) (red), or 0.8 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 66777-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL750-66777 targets TOM20 in FC (Intra) applications and shows reactivity with human samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human
Immunogen TOM20 fusion protein Ag2378 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name translocase of outer mitochondrial membrane 20 homolog (yeast)
Synonyms TOMM20, TOMM 20, tom 20, MOM19, MAS20
Calculated Molecular Weight 145 aa, 16 kDa
Observed Molecular Weight 16 kDa
GenBank Accession NumberBC000882
Gene Symbol TOM20
Gene ID (NCBI) 9804
Conjugate CoraLite® Plus 750 Fluorescent Dye
Excitation/Emission Maxima Wavelengths755 nm / 780 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ15388
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TOM20, also named as KIAA0016, belongs to the Tom20 family. It is a central component of the receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with TOM22, TOM20 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the TOM40 translocation pore. TOM20 is characterized as major docking receptors to mediate the recognition by different mechanisms.

实验方案

Product Specific Protocols
FC protocol for CL Plus 750 TOM20 antibody CL750-66777Download protocol
Standard Protocols
Click here to view our Standard Protocols