• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • FC experiment of HeLa using CL555-66777

    FC experiment of HeLa using CL555-66777

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human TOM20 (CL555-66777, Clone:1D6F5) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of HepG2 using CL555-66777

    IF Staining of HepG2 using CL555-66777

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®555 TOM20 antibody (CL555-66777, Clone: 1D6F5 ) at dilution of 1:200.

CoraLite®555-conjugated TOM20 Monoclonal antibody

TOM20 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL555-66777

产品说明书

CloneNo. 1D6F5

宿主/亚型

Mouse / IgG2b

种属反应性

human

应用

IF/ICC, FC (Intra)

别名

TOMM20, TOMM 20, tom 20, MOM19, MAS20

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®555
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy TOMM20 Ready-To-Use IHC Kit. TOMM20 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66777-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL555-66777 targets TOM20 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human
免疫原 TOM20 fusion protein Ag2378 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 translocase of outer mitochondrial membrane 20 homolog (yeast)
别名 TOMM20, TOMM 20, tom 20, MOM19, MAS20
计算分子量 145 aa, 16 kDa
观测分子量 16 kDa
GenBank蛋白编号BC000882
基因名称 TOM20
Gene ID (NCBI) 9804
RRIDAB_2919694
偶联类型 CoraLite®555 Fluorescent Dye
最大激发/发射波长557 nm / 570 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ15388
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TOM20, also named as KIAA0016, belongs to the Tom20 family. It is a central component of the receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with TOM22, TOM20 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the TOM40 translocation pore. TOM20 is characterized as major docking receptors to mediate the recognition by different mechanisms.

实验方案

Product Specific Protocols
IF protocol for CL555 TOM20 antibody CL555-66777Download protocol
FC protocol for CL555 TOM20 antibody CL555-66777Download protocol
Standard Protocols
Click here to view our Standard Protocols