Vinculin Monoclonal antibody

Vinculin Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig and More (1)

Applications

FC, IF, IHC, WB, ELISA and More (1)

Conjugate

Unconjugated

CloneNo.

2B5A7

Cat No : 66305-1-Ig

Print datasheet

Synonyms

CMD1W, Metavinculin, MVCL, VCL, Vinculin



经过测试的应用

Positive WB detected inHeLa cells, pig heart tissue, rat heart tissue, HepG2 cells, PC-3 cells, A2780 cells, SW 1990 cells, NIH/3T3 cells
Positive IHC detected inhuman breast cancer tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Positive FC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

产品信息

66305-1-Ig targets Vinculin in WB, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications FC, IF, IHC, WB, ELISA
Cited Applications CoIP, IF, IHC, WB
Tested Reactivity human, mouse, rat, pig
Cited Reactivityhuman, mouse, rat, hamster
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Vinculin fusion protein Ag24946
Full Name vinculin
Calculated molecular weight 1133 aa, 124 kDa
Observed molecular weight 117 kDa
GenBank accession numberBC039174
Gene symbol VCL
UniprotP18206
Gene ID (NCBI) 7414
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Vinculin belongs to the vinculin/alpha-catenin family. It is an actin filament (F-actin)-binding protein which involved in cell-matrix adhesion and cell-cell adhesion. Vinculin regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. It may also play important roles in cell morphology and locomotion. Vinculin is a 117-kDa, 1,066-amino-acid protein which is ubiquitously expressed. Its splice variant, metavinculin (124 kDa), is muscle-specific.

实验方案

Product Specific Protocols
WB protocol for Vinculin antibody 66305-1-IgDownload protocol
IHC protocol for Vinculin antibody 66305-1-IgDownload protocol
IF protocol for Vinculin antibody 66305-1-IgDownload protocol
FC protocol for Vinculin antibody 66305-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
hamsterWB,IF

J Mol Neurosci

The Mechanism of Rap1 Regulates N-cadherin to Control Neuronal Migration.

Authors - Ciqing Yang
humanWB

Nucleic Acids Res

BRCA1-associated R-loop affects transcription and differentiation in breast luminal epithelial cells.

Authors - Huai-Chin Chiang
humanWB

Carcinogenesis

Functional genetic variants of RUVBL1 predict overall survival of Chinese patients with epithelial ovarian cancer.

Authors - Haoran Li
ratIF

Can J Physiol Pharmacol

Focal adhesions are involved in simulated microgravity-induced basilar and femoral arterial remodelling in rats.

Authors - Min Jiang
ratIF

Heliyon

The impact of photofunctionalized gold nanoparticles on osseointegration.

Authors - Yassir Elkhidir
humanIF

Cancer Biol Ther

CRMP4a suppresses cell motility by sequestering RhoA activity in prostate cancer cells.

Authors - Changlin Li