验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, HEK-293 cells, HeLa cells |
| Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy ZC3HAV1 Ready-To-Use IHC Kit. ZC3HAV1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66413-1-Ig targets ZC3HAV1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
| Cited Applications | WB, IF |
| Tested Reactivity | human |
| Cited Reactivity | human |
| Immunogen | ZC3HAV1 fusion protein Ag10364 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | zinc finger CCCH-type, antiviral 1 |
| Synonyms | PRO1677, PARP13, Inactive Poly [ADP-ribose] polymerase 13, ARTD13, ADP-ribosyltransferase diphtheria toxin-like 13 |
| Calculated Molecular Weight | 699aa,78 kDa; 902aa,101 kDa |
| Observed Molecular Weight | 100 kDa |
| GenBank Accession Number | BC025308 |
| Gene Symbol | ZC3HAV1 |
| Gene ID (NCBI) | 56829 |
| RRID | AB_2881785 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q7Z2W4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
he zinc-finger antiviral protein (ZAP) was originally identifed from a rat cDNA library due to its conferring resistance to retroviruses. ZC3HAV1 is a CCCH-type zinc finger protein, it may primarily function to inhibit viral gene expression and induce an innate immunity to viral infection. Alternative splicing occurs at this locus and two variants, each encoding distinct isoforms.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ZC3HAV1 antibody 66413-1-Ig | Download protocol |
| IHC protocol for ZC3HAV1 antibody 66413-1-Ig | Download protocol |
| IF protocol for ZC3HAV1 antibody 66413-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
mBio The Zinc Finger Antiviral Protein ZAP Restricts Human Cytomegalovirus and Selectively Binds and Destabilizes Viral UL4/UL5 Transcripts.
| ||
J Virol Riplet Binds the Zinc Finger Antiviral Protein (ZAP) and Augments ZAP-Mediated Restriction of HIV-1. | ||
EMBO Rep Interferon inhibits a model RNA virus via a limited set of inducible effector genes | ||
