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ZC3HAV1 Monoclonal antibody

ZC3HAV1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66413-1-Ig

产品说明书

CloneNo. 1G10B9

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA and More (1)

PRO1677, PARP13, Inactive Poly [ADP-ribose] polymerase 13, ARTD13, ADP-ribosyltransferase diphtheria toxin-like 13

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inJurkat cells, HEK-293 cells, HeLa cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy ZC3HAV1 Ready-To-Use IHC Kit. ZC3HAV1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66413-1-Ig targets ZC3HAV1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human
文献引用反应性human
免疫原 ZC3HAV1 fusion protein Ag10364 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 zinc finger CCCH-type, antiviral 1
别名 PRO1677, PARP13, Inactive Poly [ADP-ribose] polymerase 13, ARTD13, ADP-ribosyltransferase diphtheria toxin-like 13
计算分子量699aa,78 kDa; 902aa,101 kDa
观测分子量 100 kDa
GenBank蛋白编号BC025308
基因名称 ZC3HAV1
Gene ID (NCBI) 56829
RRIDAB_2881785
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ7Z2W4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

he zinc-finger antiviral protein (ZAP) was originally identifed from a rat cDNA library due to its conferring resistance to retroviruses. ZC3HAV1 is a CCCH-type zinc finger protein, it may primarily function to inhibit viral gene expression and induce an innate immunity to viral infection. Alternative splicing occurs at this locus and two variants, each encoding distinct isoforms.

实验方案

Product Specific Protocols
WB protocol for ZC3HAV1 antibody 66413-1-IgDownload protocol
IHC protocol for ZC3HAV1 antibody 66413-1-IgDownload protocol
IF protocol for ZC3HAV1 antibody 66413-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

mBio

The Zinc Finger Antiviral Protein ZAP Restricts Human Cytomegalovirus and Selectively Binds and Destabilizes Viral UL4/UL5 Transcripts.

Authors - Ana Cristina Gonzalez-Perez
  • KO Validated
humanIF

J Virol

Riplet Binds the Zinc Finger Antiviral Protein (ZAP) and Augments ZAP-Mediated Restriction of HIV-1.

Authors - Marlene V Buckmaster
humanWB

EMBO Rep

Interferon inhibits a model RNA virus via a limited set of inducible effector genes

Authors - Matthew B McDougal
humanWB

Nat Commun

TRIM25 predominately associates with anti-viral stress granules

Authors - Zehua Shang
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