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验证数据展示

  • IF Staining of HepG2 using CL594-66413

    IF Staining of HepG2 using CL594-66413

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CL594-66413 (ZC3HAV1 antibody) at dilution of 1:100.

  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated ZC3HAV1 Monoclonal antibody

ZC3HAV1 Monoclonal Antibody for IF/ICC
Cat No. CL594-66413

Print datasheet

CloneNo. 1G10B9

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF/ICC

Synonyms

DKFZp686F2052, DKFZp686H1869, DKFZp686O19171, FLB6421, FLJ13288, ZAP, ZC3H2, ZC3HAV1, ZC3HDC2, Zinc finger antiviral protein

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy ZC3HAV1 Ready-To-Use IHC Kit. ZC3HAV1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66413-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66413 targets ZC3HAV1 in IF/ICC applications and shows reactivity with human samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human
Immunogen ZC3HAV1 fusion protein Ag10364 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name zinc finger CCCH-type, antiviral 1
Synonyms DKFZp686F2052, DKFZp686H1869, DKFZp686O19171, FLB6421, FLJ13288, ZAP, ZC3H2, ZC3HAV1, ZC3HDC2, Zinc finger antiviral protein
Calculated Molecular Weight699aa,78 kDa; 902aa,101 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberBC025308
Gene Symbol ZC3HAV1
Gene ID (NCBI) 56829
RRIDAB_2883579
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ7Z2W4
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

he zinc-finger antiviral protein (ZAP) was originally identifed from a rat cDNA library due to its conferring resistance to retroviruses. ZC3HAV1 is a CCCH-type zinc finger protein, it may primarily function to inhibit viral gene expression and induce an innate immunity to viral infection. Alternative splicing occurs at this locus and two variants, each encoding distinct isoforms.

实验方案

Product Specific Protocols
IF protocol for CL594 ZC3HAV1 antibody CL594-66413Download protocol
Standard Protocols
Click here to view our Standard Protocols