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验证数据展示

  • IF Staining of HepG2 using CL594-66413

    IF Staining of HepG2 using CL594-66413

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CL594-66413 (ZC3HAV1 antibody) at dilution of 1:100.

  • Featured Product
  • KD/KO验证

CoraLite®594-conjugated ZC3HAV1 Monoclonal antibody

ZC3HAV1 Monoclonal Antibody for IF/ICC
Cat No. CL594-66413

产品说明书

CloneNo. 1G10B9

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

IF/ICC

别名

DKFZp686F2052, DKFZp686H1869, DKFZp686O19171, FLB6421, FLJ13288, ZAP, ZC3H2, ZC3HAV1, ZC3HDC2, Zinc finger antiviral protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy ZC3HAV1 Ready-To-Use IHC Kit. ZC3HAV1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66413-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66413 targets ZC3HAV1 in IF/ICC applications and shows reactivity with human samples.

经测试应用 IF/ICC Application Description
经测试反应性 human
免疫原 ZC3HAV1 fusion protein Ag10364 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 zinc finger CCCH-type, antiviral 1
别名 DKFZp686F2052, DKFZp686H1869, DKFZp686O19171, FLB6421, FLJ13288, ZAP, ZC3H2, ZC3HAV1, ZC3HDC2, Zinc finger antiviral protein
计算分子量699aa,78 kDa; 902aa,101 kDa
观测分子量 100 kDa
GenBank蛋白编号BC025308
基因名称 ZC3HAV1
Gene ID (NCBI) 56829
RRIDAB_2883579
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ7Z2W4
储存缓冲液 PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

he zinc-finger antiviral protein (ZAP) was originally identifed from a rat cDNA library due to its conferring resistance to retroviruses. ZC3HAV1 is a CCCH-type zinc finger protein, it may primarily function to inhibit viral gene expression and induce an innate immunity to viral infection. Alternative splicing occurs at this locus and two variants, each encoding distinct isoforms.

实验方案

Product Specific Protocols
IF protocol for CL594 ZC3HAV1 antibody CL594-66413Download protocol
Standard Protocols
Click here to view our Standard Protocols