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验证数据展示

  • FC experiment of MCF-7 using CL488-60315

    FC experiment of MCF-7 using CL488-60315

    1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human c-SRC (CL488-60315, Clone:5E10C4) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL488-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated c-SRC Monoclonal antibody

c-SRC Monoclonal Antibody for FC (Intra)
Cat No. CL488-60315

Print datasheet

CloneNo. 5E10C4

Host / Isotype

Mouse / IgG2b

Reactivity

human, hamster, mouse

Applications

FC (Intra)

Synonyms

SRC, pp60c-src, p60-Src, EC:2.7.10.2, cSRC

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy c-SRC Ready-To-Use IHC Kit. c-SRC primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 60315-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-60315 targets c-SRC in FC (Intra) applications and shows reactivity with human, hamster, mouse samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human, hamster, mouse
Immunogen c-SRC fusion protein Ag22306 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian)
Synonyms SRC, pp60c-src, p60-Src, EC:2.7.10.2, cSRC
Calculated Molecular Weight 60 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberBC011566
Gene Symbol SRC
Gene ID (NCBI) 6714
ENSEMBL Gene IDENSG00000197122
RRIDAB_2934431
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP12931
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SRC, also named as SRC1 and p60-Src, belongs to the protein kinase superfamily, Tyr protein kinase family and SRC subfamily. It is a non-receptor protein tyrosine kinase that plays pivotal roles in numerous cellular processes such as proliferation, migration, and transformation. In concert with PTK2B, SRC plays an important role in osteoclastic bone resorption. It promotes energy production in osteoclasts by activating mitochondrial cytochrome C oxidase.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 c-SRC antibody CL488-60315Download protocol
Standard Protocols
Click here to view our Standard Protocols