CoraLite® Plus 488-conjugated c-SRC Monoclonal antibody

c-SRC Monoclonal Antibody for FC (Intra)
Cat No. CL488-60315

产品说明书

CloneNo. 5E10C4

宿主/亚型

Mouse / IgG2b

种属反应性

human, hamster, mouse

应用

FC (Intra)

SRC, pp60c-src, p60-Src, EC:2.7.10.2, cSRC

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -


经过测试的应用

Positive FC (Intra) detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy c-SRC Ready-To-Use IHC Kit. c-SRC primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 60315-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-60315 targets c-SRC in FC (Intra) applications and shows reactivity with human, hamster, mouse samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human, hamster, mouse
免疫原 c-SRC fusion protein Ag22306 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian)
别名 SRC, pp60c-src, p60-Src, EC:2.7.10.2, cSRC
计算分子量 60 kDa
观测分子量 60 kDa
GenBank蛋白编号BC011566
基因名称 SRC
Gene ID (NCBI) 6714
ENSEMBL Gene IDENSG00000197122
RRIDAB_2934431
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP12931
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SRC, also named as SRC1 and p60-Src, belongs to the protein kinase superfamily, Tyr protein kinase family and SRC subfamily. It is a non-receptor protein tyrosine kinase that plays pivotal roles in numerous cellular processes such as proliferation, migration, and transformation. In concert with PTK2B, SRC plays an important role in osteoclastic bone resorption. It promotes energy production in osteoclasts by activating mitochondrial cytochrome C oxidase.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 c-SRC antibody CL488-60315Download protocol
Standard Protocols
Click here to view our Standard Protocols
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