验证数据展示
经过测试的应用
| Positive IF/ICC detected in | MCF-7 cells |
| Positive FC (Intra) detected in | MCF-7 cells |
Planning an IHC experiment? We recommend our IHCeasy c-SRC Ready-To-Use IHC Kit. c-SRC primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 60315-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 1 publications below |
产品信息
CL594-60315 targets c-SRC in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, hamster samples.
| Tested Applications | IF/ICC, FC (Intra) Application Description |
| Cited Applications | IF |
| Tested Reactivity | human, mouse, hamster |
| Cited Reactivity | human |
| Immunogen | c-SRC fusion protein Ag22306 种属同源性预测 |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) |
| Synonyms | SRC, pp60c-src, p60-Src, EC:2.7.10.2, cSRC |
| Calculated Molecular Weight | 60 kDa |
| Observed Molecular Weight | 60 kDa |
| GenBank Accession Number | BC011566 |
| Gene Symbol | SRC |
| Gene ID (NCBI) | 6714 |
| ENSEMBL Gene ID | ENSG00000197122 |
| RRID | AB_2883461 |
| Conjugate | CoraLite®594 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 588 nm / 604 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P12931 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SRC, also named as SRC1 and p60-Src, belongs to the protein kinase superfamily, Tyr protein kinase family and SRC subfamily. It is a non-receptor protein tyrosine kinase that plays pivotal roles in numerous cellular processes such as proliferation, migration, and transformation. In concert with PTK2B, SRC plays an important role in osteoclastic bone resorption. It promotes energy production in osteoclasts by activating mitochondrial cytochrome C oxidase.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 c-SRC antibody CL594-60315 | Download protocol |
| FC protocol for CL594 c-SRC antibody CL594-60315 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
