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CoraLite®594-conjugated c-SRC Monoclonal antibody

c-SRC Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, hamster

Applications

IF/ICC, FC (Intra) and More (1)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

5E10C4

Cat no : CL594-60315

Print datasheet

Synonyms

SRC, pp60c-src, p60-Src, EC:2.7.10.2, cSRC

验证数据展示

  • IF Staining of MCF-7 using CL594-60315

    IF Staining of MCF-7 using CL594-60315

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CL594-60315 (c-SRC antibody) at dilution of 1:100.

  • IF Staining of MCF-7 using CL594-60315

    IF Staining of MCF-7 using CL594-60315

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CoraLite®594 c-SRC antibody (CL594-60315, Clone: 5E10C4 ) at dilution of 1:200.

  • IF Staining of MCF-7 using CL594-60315

    IF Staining of MCF-7 using CL594-60315

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CoraLite®594 c-SRC antibody (CL594-60315, Clone: 5E10C4 ) at dilution of 1:100.

  • FC experiment of MCF-7 using CL594-60315

    FC experiment of MCF-7 using CL594-60315

    1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human c-SRC (CL594-60315, Clone:5E10C4) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL594-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of human breast cancer using CL594-60315

    IF Staining of human breast cancer using CL594-60315

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CoraLite®594-conjugated c-SRC antibody (CL594-60315, Clone: 5E10C4 ) at dilution of 1:100.

经过测试的应用

Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy c-SRC Ready-To-Use IHC Kit. c-SRC primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 60315-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

CL594-60315 targets c-SRC in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, hamster samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Cited ApplicationsIF
Tested Reactivity human, mouse, hamster
Cited Reactivityhuman
Immunogen c-SRC fusion protein Ag22306 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian)
Synonyms SRC, pp60c-src, p60-Src, EC:2.7.10.2, cSRC
Calculated Molecular Weight 60 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberBC011566
Gene Symbol SRC
Gene ID (NCBI) 6714
ENSEMBL Gene IDENSG00000197122
RRIDAB_2883461
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP12931
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SRC, also named as SRC1 and p60-Src, belongs to the protein kinase superfamily, Tyr protein kinase family and SRC subfamily. It is a non-receptor protein tyrosine kinase that plays pivotal roles in numerous cellular processes such as proliferation, migration, and transformation. In concert with PTK2B, SRC plays an important role in osteoclastic bone resorption. It promotes energy production in osteoclasts by activating mitochondrial cytochrome C oxidase.

实验方案

Product Specific Protocols
IF protocol for CL594 c-SRC antibody CL594-60315Download protocol
FC protocol for CL594 c-SRC antibody CL594-60315Download protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Mol Cancer

Cholesterol promotes EGFR-TKIs resistance in NSCLC by inducing EGFR/Src/Erk/SP1 signaling-mediated ERRα re-expression.

Authors - Zhenzhen Pan
View Article