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- KD/KO Validated
CoraLite® Plus 488-conjugated iNOS Polyclonal antibody
iNOS Polyclonal Antibody for IF/ICC, FC (Intra)
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF/ICC, FC (Intra) and More (1)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | HepG2 cells |
For other applications, we recommend the unconjugated version of this antibody, 18985-1-AP
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 1 publications below |
产品信息
CL488-18985 targets iNOS in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | IF/ICC, FC (Intra) Application Description |
| Cited Applications | IF |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Immunogen | Peptide 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | nitric oxide synthase 2, inducible |
| Synonyms | NOS2, NOS type II, Inducible NOS, Inducible NO synthase, HEP-NOS |
| Calculated Molecular Weight | 131 kDa |
| Observed Molecular Weight | 110-130 kDa, 65-70 kDa |
| GenBank Accession Number | NM_000625 |
| Gene Symbol | iNOS |
| Gene ID (NCBI) | 4843 |
| RRID | AB_2919161 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P35228 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NOS2, also named as iNOS and NOS2A, produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. NO is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission, antimicrobial and antitumoral activities. NOS2 is a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. iNOS has a very short half-life due to rapid degradation by calpain. iNOS monomer is a direct substrate of calpain I and can be cleaved by calpain I at the canonical CaM-binding site(503-532aa) of iNOS, and then a ~70kD band can be detected by western (PMID:11786228). This antibody is specific to NOS2.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 iNOS antibody CL488-18985 | Download protocol |
| FC protocol for CL Plus 488 iNOS antibody CL488-18985 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Neural Regen Res Small extracellular vesicles from hypoxia-preconditioned bone marrow mesenchymal stem cells attenuate spinal cord injury via miR-146a-5p-mediated regulation of macrophage polarization | ||
J Biochem Mol Toxicol KLF4-induced upregulation of SOCS1 ameliorates myocardial ischemia/reperfusion injury by attenuating AC16 cardiomyocyte damage and enhancing M2 macrophage polarization | ||
