Validation Data Gallery
Product Information
mNeonGreen-Trap Agarose is an affinity reagent for IP of mNeonGreen-fusion proteins. It consists of a mNeonGreen Nanobody/ VHH coupled to agarose beads.
| Description | Immunoprecipitation of mNeonGreen-fusion proteins and their interacting factors with anti-mNeonGreen Nanobody conjugated to beads.
• Fast, reliable & efficient one-step immunoprecipitation • Ready-to-use • No heavy & light antibody chains • Stable under harsh washing conditions • Suitable for downstream mass spec analysis • Short incubation time of about 60 minutes |
| Applications | IP, CoIP, ChIP, RIP |
| Specificity/Target | mNeonGreen For the complete list, please click here: Fluorescent protein specificity table |
| Binding capacity | 30 μg of recombinant mNeonGreen per 25 μL bead slurry |
| Conjugate | Agarose beads; bead size: ~ 90 µm (cross-linked 4 % agarose beads) |
| Elution buffer | SDS sample buffer 0.2 M glycine pH 2.5 |
| Wash buffer compatibility | 4 M urea, 2 M NaCl, 10 mM DTT, 2 % Nonidet P40 Substitute, 1 % Triton X-100, 0.2 % SDS |
| Type | Nanobody |
| Class | Recombinant |
| Host | Alpaca |
| Affinity (KD) | Dissociation constant KD of 2 nM |
| Compatibility with mass spectrometry | The mNeonGreen-Trap is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry |
| RRID | AB_2827593 |
| Storage Buffer | 20% EtOH |
| Storage Condition | Upon receipt store at +4°C. Do not freeze! |
| Size | 25ul/reactions (eg：20rxns=500ul slurry) |
Documentation
| SDS |
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| nta_SDS_mNeonGreen-Trap Agarose (EN) |
| Datasheet |
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| Protocol mNeonGreen-Trap Agarose (PDF) |
| Trouble shooting |
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| Troubleshooting guide immunoprecipitation (IP) |
| Fluorescent Specificity |
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| Fluorescent protein specificity table |
| Split Technology |
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| Split Fluorescent Protein Technology (PDF) |
Publications
| Application | Title |
|---|---|
Cell Stem Cell Vimentin Coordinates Protein Turnover at the Aggresome during Neural Stem Cell Quiescence Exit. | |
Proc Natl Acad Sci U S A The Bardet-Biedl syndrome protein complex is an adapter expanding the cargo range of intraflagellar transport trains for ciliary export. | |
J Cell Biol SGEF forms a complex with Scribble and Dlg1 and regulates epithelial junctions and contractility. | |
Plant J Elucidation of the late steps in the glycan-dependent ERAD of soluble misfolded glycoproteins |