ChromoTek mNeonGreen-Trap Agarose beads
mNeonGreen-Trap Agarose is an affinity reagent for IP of mNeonGreen-fusion proteins. It consists of a mNeonGreen Nanobody/ VHH coupled to agarose beads.
Cat No : nta
Synonyms
Validation Data Gallery
Product Information
mNeonGreen-Trap Agarose is an affinity reagent for IP of mNeonGreen-fusion proteins. It consists of a mNeonGreen Nanobody/ VHH coupled to agarose beads.
| Applications | IP, CoIP, ChIP, RIP |
| Clonality | Monoclonal |
| Host | Alpaca |
| Affinity | Dissociation constant KD of 2 nM |
| Specificity/Target | mNeonGreen, Branchiostoma lanceolatum |
| Coupled VHH | Recombinant, monoclonal anti-mNeonGreen single domain antibody (sdAb) fragment |
| Conjugate | Agarose |
| Beads | ~ 90 µm (cross-linked 4 % agarose beads) |
| Binding capacity | 10 µL slurry bind approximately 12 µg of recombinant mNeonGreen |
| Elution buffer | SDS sample buffer 0.2 M glycine pH 2.5 |
| Storage Buffer | 20% EtOH |
| Storage Condition | Upon receipt store at +4°C. Do not freeze! |
Documentation
| SDS |
|---|
| Split Fluorescent Protein Technology (PDF) |
| SDS mNeonGreen-Trap Agarose |
| Protocol |
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| Protocol mNeonGreen-Trap Agarose (PDF) |
| Trouble shooting |
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| Troubleshooting guide immunoprecipitation (IP) |
| Brochure |
|---|
| Product brochure mNeonGreen (PDF) |
Publications
| Application | Title |
|---|---|
Cell Stem Cell Vimentin Coordinates Protein Turnover at the Aggresome during Neural Stem Cell Quiescence Exit. | |
J Fungi (Basel) Structure of the Yeast Cell Wall Integrity Sensor Wsc1 Reveals an Essential Role of Surface-Exposed Aromatic Clusters. | |
J Cell Biol SGEF forms a complex with Scribble and Dlg1 and regulates epithelial junctions and contractility. | |
Proc Natl Acad Sci U S A The Bardet-Biedl syndrome protein complex is an adapter expanding the cargo range of intraflagellar transport trains for ciliary export. | |