ChromoTek mNeonGreen-Trap Magnetic Agarose, kit

The ChromoTek mNeonGreen-Trap Magnetic Agarose is an affinity resin for IP of mNeonGreen-fusion proteins. It consists of a mNeonGreen Nanobody/ VHH coupled to magnetic agarose beads.

Specificity

mNeonGreen

Applications

IP, CoIP, ChIP, RIP

Type

Nanobody

Conjugate

Magnetic agarose

Cat No : ntmak

Synonyms

mNeonGreen-Trap, mNeonGreen, multimeric yellow fluorescence protein, mNeonGreen tag



Product Information

The ChromoTek mNeonGreen-Trap Magnetic Agarose is an affinity resin for IP of mNeonGreen-fusion proteins. It consists of a mNeonGreen Nanobody/ VHH coupled to magnetic agarose beads.

DescriptionThe GFP-Trap Magnetic Agarose Kit contains GFP-Trap Magnetic Agarose, lysis, wash, and elution buffers for efficient immunoprecipitation of GFP-fusion proteins and their interacting factors.

• Fast, reliable & efficient one-step immunoprecipitation

• Ready-to-use

• No heavy & light antibody chains on the SDS-PAGE

• Stable under harsh washing conditions

• Suitable for downstream mass spec analysis

ApplicationsIP, CoIP, ChIP, RIP
Specificity/TargetmNeonGreen
Binding capacity30 μg of recombinant mNeonGreen per 25 μL bead slurry
ConjugateMagnetic agarose beads; bead size: ~40 µm (cross-linked 6 % magnetic agarose beads)
Elution bufferSDS sample buffer
0.2 M glycine pH 2.5
Wash buffer compatibility4 M urea, 2 M NaCl, 10 mM DTT, 2 % Nonidet P40 Substitute, 1 % Triton X-100, 0.2 % SDS
Type Nanobody
ClassRecombinant
HostAlpaca
Affinity (KD) Dissociation constant KD of 2 nM
Compatibility with mass spectrometryThe mNeonGreen-Trap is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry
RRIDAB_2827594
Storage Buffer20% ethanol
Storage ConditionShipped at ambient temperature. Upon receipt store at 4°C. Stable for one year. Do not freeze!

Kit components

ComponentDescription
mNeonGreen-Trap Magnetic Agarose20 reactions (500 µL)
Lysis bufferOptimized for cytoplasmatic proteins and mammalian cell lysis
RIPA bufferOptimized for nuclear/chromatin proteins and mammalian cell lysis
Wash buffer Removal of unwanted proteins, peptides, etc.
Dilution bufferDilution of cell lysate
Elution bufferFor acidic elution
{{ptg:OtherFormatInfo}}