ChromoTek mNeonGreen-Trap Magnetic Agarose, kit

The ChromoTek mNeonGreen-Trap Magnetic Agarose is an affinity resin for IP of mNeonGreen-fusion proteins. It consists of a mNeonGreen Nanobody/ VHH coupled to magnetic agarose beads.

Specificity

mNeonGreen

Applications

IP, CoIP, ChIP, RIP

Conjugate

Magnetic Agarose

Cat No : ntmak

Synonyms

mNeonGreen-Trap, mNeonGreen, multimeric yellow fluorescence protein, mNeonGreen tag



Product Information

The ChromoTek mNeonGreen-Trap Magnetic Agarose is an affinity resin for IP of mNeonGreen-fusion proteins. It consists of a mNeonGreen Nanobody/ VHH coupled to magnetic agarose beads.

ApplicationsIP, CoIP, ChIP, RIP
ClonalityMonoclonal
HostAlpaca
AffinityDissociation constant KD of 2 nM
Specificity/TargetmNeonGreen, Branchiostoma lanceolatum
Coupled VHHrecombinant, monoclonal anti-mNeonGreen single domain antibody (sdAb) fragment
ConjugateMagnetic Agarose
Beads~ 40 µm
Binding capacity10 µL slurry bind more than 12 µg of recombinant mNeonGreen
Elution bufferSDS sample buffer
0.2 M glycine pH 2.5
Storage Buffer20% EtOH
Storage ConditionUpon receipt store at +4°C. Do not freeze!

Kit components

ComponentDescription
mNeonGreen-Trap Magnetic Agarose20 reactions (500 µL)
Lysis bufferOptimized for cytoplasmatic proteins and mammalian cell lysis
RIPA bufferOptimized for nuclear/chromatin proteins and mammalian cell lysis
Wash buffer Removal of unwanted proteins, peptides, etc.
Dilution bufferDilution of cell lysate
Elution bufferFor acidic elution