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验证数据展示

  • IF Staining of HeLa using CL488-13509

    IF Staining of HeLa using CL488-13509

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 p115, USO1 antibody (CL488-13509) at dilution of 1:200.

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated p115, USO1 Polyclonal antibody

p115, USO1 Polyclonal Antibody for IF/ICC
Cat No. CL488-13509

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC

别名

P115, p115, USO1, Protein USO1 homolog, TAP, USO1, VDP, Vesicle docking protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy USO1 Ready-To-Use IHC Kit. USO1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 13509-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-13509 targets p115, USO1 in IF/ICC applications and shows reactivity with human samples.

经测试应用 IF/ICC Application Description
经测试反应性 human
免疫原 p115, USO1 fusion protein Ag4431 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 USO1 homolog, vesicle docking protein (yeast)
别名 P115, p115, USO1, Protein USO1 homolog, TAP, USO1, VDP, Vesicle docking protein
计算分子量 962 aa, 108 kDa
GenBank蛋白编号BC032654
基因名称 USO1
Gene ID (NCBI) 8615
RRIDAB_2919074
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO60763
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

p115, also known as USO1, TAP (transcytosis-associated protein) or VDP (vesicle docking protein) is a general vesicular transport factor and plays an important role at different steps of vesicular transport. It is a 962-residue peripheral membrane protein which recycles between the cytosol and the Golgi apparatus during interphase (PMID: 9478999). p115 forms stable homodimers (PMID: 19247479). Rab1 recruits p115 to coat protein complex II (COPII) vesicles during budding from the endoplasmic reticulum, where p115 interacts directly with a select set of SNARE proteins (PMID: 10903204). p115 is required for intra-Golgi transport, and also functions in endoplasmic reticulum to Golgi trafficking, Golgi biogenesis and exocytotic transport (PMID: 19247479).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 p115, USO1 antibody CL488-13509Download protocol
Standard Protocols
Click here to view our Standard Protocols