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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated p115, USO1 Polyclonal antibody

p115, USO1 Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

Cat no : CL594-13509

Print datasheet

Synonyms

P115, p115, USO1, Protein USO1 homolog, TAP, USO1, VDP, Vesicle docking protein

验证数据展示

  • IF Staining of HeLa using CL594-13509

    IF Staining of HeLa using CL594-13509

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®594 p115, USO1 antibody (CL594-13509) at dilution of 1:200, 488-phalloidin (green).

经过测试的应用

Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy USO1 Ready-To-Use IHC Kit. USO1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 13509-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-13509 targets p115, USO1 in IF/ICC applications and shows reactivity with human samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human
Immunogen p115, USO1 fusion protein Ag4431 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name USO1 homolog, vesicle docking protein (yeast)
Synonyms P115, p115, USO1, Protein USO1 homolog, TAP, USO1, VDP, Vesicle docking protein
Calculated Molecular Weight 962 aa, 108 kDa
GenBank Accession NumberBC032654
Gene Symbol USO1
Gene ID (NCBI) 8615
RRIDAB_2919797
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO60763
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

p115, also known as USO1, TAP (transcytosis-associated protein) or VDP (vesicle docking protein) is a general vesicular transport factor and plays an important role at different steps of vesicular transport. It is a 962-residue peripheral membrane protein which recycles between the cytosol and the Golgi apparatus during interphase (PMID: 9478999). p115 forms stable homodimers (PMID: 19247479). Rab1 recruits p115 to coat protein complex II (COPII) vesicles during budding from the endoplasmic reticulum, where p115 interacts directly with a select set of SNARE proteins (PMID: 10903204). p115 is required for intra-Golgi transport, and also functions in endoplasmic reticulum to Golgi trafficking, Golgi biogenesis and exocytotic transport (PMID: 19247479).

实验方案

Product Specific Protocols
IF protocol for CL594 p115, USO1 antibody CL594-13509Download protocol
Standard Protocols
Click here to view our Standard Protocols